Assessment of two immunoassays for detection of IgM antibodies to scrub typhus using a serum panel.
ELISA
Immunochromatography (ICT)
Rapid diagnosis
Resource poor setting
Scrub typhus
Journal
Indian journal of medical microbiology
ISSN: 1998-3646
Titre abrégé: Indian J Med Microbiol
Pays: United States
ID NLM: 8700903
Informations de publication
Date de publication:
Historique:
entrez:
22
5
2020
pubmed:
22
5
2020
medline:
30
7
2020
Statut:
ppublish
Résumé
Laboratory tests are necessary for diagnosis of scrub typhus (ST) especially in the absence of the distinctive eschar. Performance of an ELISA and ICT (immunochromatography) to detect IgM antibodies to scrub typhus was assessed using a panel of 346 sera chosen from healthy individuals, those with scrub typhus and scrub-typhus like illness. A sensitivity of 98.7% for ST IgM ICT and 97.4% for ST IgM ELISA was observed while specificity was 96.3% for ICT and 95.9% for ELISA. As excellent concordance (98.8%) was noted between the two assays, IgM ICT can be used for rapid diagnosis of scrub typhus. Abbreviations: ST IgM ELISA: Scrub typhus IgM ELISA; ST IgM ICT: Scrub Typhus IgM Immunochromatography, Rapid diagnostic test: RDT.
Identifiants
pubmed: 32436884
pii: IndianJMedMicrobiol_2019_37_4_584_284522
doi: 10.4103/ijmm.IJMM_20_130
doi:
Substances chimiques
Antibodies
0
Immunoglobulin M
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
584-586Déclaration de conflit d'intérêts
None