Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
18
12
2019
accepted:
06
05
2020
entrez:
22
5
2020
pubmed:
22
5
2020
medline:
12
8
2020
Statut:
epublish
Résumé
Vitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitrectomy surgery (n = 44, mean age: 56.09±10.2 years) were recruited. Dry eye evaluation was done before and 8 weeks after surgery (2 weeks after omitting topical eye drops). Conjunctival imprint cytology for goblet cell count and tear Mucin 5AC (MUC5AC) protein estimation was done. Gene expressions of MUC5AC, MUC4, MUC16, Aquaporin 4 (AQP4) and AQP5 were analyzed in the conjunctival imprint cells by qPCR. None of the patients exhibited clinical signs of dry eye after VR surgery. But the conjunctival goblet cell density (GCD) was significantly lowered post-VR surgery (63% cases, **p = 0.012) with no alterations in the tear MUC5AC protein. Post-VR surgery, the conjunctival cell gene expression of MUC4, MUC16 and AQP4 were significantly increased (*p = 0.025, *p = 0.05 and *p = 0.02 respectively) and AQP5 was significantly lowered (*p = 0.037), with no change in MUC5AC expression. Tear cytokines were significantly increased post-VR surgery (anti-inflammatory: IL1RA, IL4, IL5, IL9, FGF; PDGFbb and pro-inflammatory: IL2, IL6, IL15, GMCSF and IFNg). Though clinical signs of dry eye were not observed after VR surgery, ocular surface changes in the form of reduced GCD, altered MUC5AC, MUC4, MUC16, AQP4, AQP5 and cytokines are suggestive of dry eye outcome at the molecular level especially inpatients aged above 51 years, especially female gender and those who are diabetic.
Identifiants
pubmed: 32437405
doi: 10.1371/journal.pone.0233517
pii: PONE-D-19-34984
pmc: PMC7241722
doi:
Substances chimiques
AQP4 protein, human
0
Aquaporin 4
0
Aquaporin 5
0
Aquaporins
0
CA-125 Antigen
0
MUC16 protein, human
0
MUC4 protein, human
0
Membrane Proteins
0
Mucin 5AC
0
Mucin-4
0
Mucins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0233517Déclaration de conflit d'intérêts
The authors have read the journal’s policy and have the following conflicts: Novartis Healthcare Private Limited funded this project. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.
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