Assessment of the Presence of Hepatitis E virus in Surface Water and Drinking Water in Portugal.
Hepatitis E virus
RT-qPCR
Vero E6 cell line
drinking water
enteric viruses
surface water
viral infectivity
water quality
water treatment
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
19 May 2020
19 May 2020
Historique:
received:
01
05
2020
revised:
15
05
2020
accepted:
18
05
2020
entrez:
23
5
2020
pubmed:
23
5
2020
medline:
23
5
2020
Statut:
epublish
Résumé
Hepatitis E virus (HEV) is a non-enveloped single-stranded positive-sense RNA virus, belonging to the Hepeviridae family, resistant to environmental conditions, and transmitted by the consumption of contaminated water. This virus is responsible for both sporadic and epidemic outbreaks, leading to thousands of infections per year in several countries, and is thus considered an emerging disease in Europe and Asia. This study refers to a survey in Portugal during 2019, targeting the detection and eventual quantification of enteric viruses in samples from surface and drinking water. Samples positive for HEV RNA were recurrently found by reverse transcription quantitative PCR (RT-qPCR), in both types of matrix. The infectivity of these samples was evaluated in cultured Vero E6 cells and RNA from putative viruses produced in cultures evidencing cytopathic effects and was subjected to RT-qPCR targeting HEV genomic RNA. Our results evidenced the existence of samples positive either for HEV RNA (77.8% in surface water and 66.7% in drinking water) or for infectious HEV (23.0% in surface water and 27.7% in drinking water). These results highlight the need for effective virological control of water for human consumption and activities.
Identifiants
pubmed: 32438739
pii: microorganisms8050761
doi: 10.3390/microorganisms8050761
pmc: PMC7285264
pii:
doi:
Types de publication
Journal Article
Langues
eng
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Water Res. 2017 Dec 15;127:41-49
pubmed: 29031798
Water Res. 2018 May 15;135:168-186
pubmed: 29471200
PLoS One. 2017 May 16;12(5):e0177525
pubmed: 28520759
Zoonoses Public Health. 2012 Nov;59(7):477-81
pubmed: 22583975
Int J Hyg Environ Health. 2016 Jul;219(4-5):405-11
pubmed: 27079972
Int J Infect Dis. 2019 Mar;80:66-72
pubmed: 30639406
Aust N Z J Public Health. 2012 Jun;36(3):205-7
pubmed: 22672023
J Appl Microbiol. 2010 Mar;108(3):1023-1031
pubmed: 19761463
Appl Environ Microbiol. 2014 Nov;80(21):6771-81
pubmed: 25172863
Front Microbiol. 2016 Aug 03;7:1183
pubmed: 27536278
Ann Agric Environ Med. 2018 Jun 20;25(2):364-367
pubmed: 29936796
Sci Total Environ. 2018 Sep 1;634:1174-1183
pubmed: 29710623
Liver Int. 2014 Feb;34 Suppl 1:29-37
pubmed: 24373076
Viruses. 2018 May 25;10(6):
pubmed: 29799485
Int J Food Microbiol. 2018 Jul 20;277:34-40
pubmed: 29680694
Viruses. 2019 Mar 13;11(3):
pubmed: 30871152
J Vet Sci. 2016 Mar;17(1):1-11
pubmed: 27051334
Ann Agric Environ Med. 2015;22(4):661-4
pubmed: 26706972
Viruses. 2019 Sep 20;11(10):
pubmed: 31547135
Appl Environ Microbiol. 2007 Dec;73(24):7891-7
pubmed: 17933913
J Appl Microbiol. 2001 Dec;91(6):945-62
pubmed: 11851802
Curr Protoc Microbiol. 2008 Nov;Appendix 4:Appendix 4E
pubmed: 19016439
Appl Environ Microbiol. 2013 Feb;79(4):1342-52
pubmed: 23263948
BMC Vet Res. 2020 Mar 21;16(1):95
pubmed: 32199460
Food Environ Virol. 2012 Jun;4(2):41-67
pubmed: 23412811
Water Res. 2002 Jan;36(1):248-56
pubmed: 11766801
Int J Food Microbiol. 2015 Jun 2;202:57-65
pubmed: 25771512
Euroasian J Hepatogastroenterol. 2017 Jan-Jun;7(1):73-77
pubmed: 29201777
Appl Environ Microbiol. 2005 May;71(5):2403-11
pubmed: 15870327
J Appl Microbiol. 2016 Oct;121(4):1180-8
pubmed: 27377539
Appl Environ Microbiol. 2010 Sep;76(17):5870-3
pubmed: 20601505
Appl Environ Microbiol. 2009 Jan;75(2):297-307
pubmed: 19011062
Water Res. 2020 Jan 1;168:115141
pubmed: 31590036
J Virol Methods. 2013 Jul;191(1):16-23
pubmed: 23578702
Front Microbiol. 2020 Jan 14;10:2937
pubmed: 31993027
FEMS Microbiol Rev. 2012 Jul;36(4):786-814
pubmed: 22091646
J Vis Exp. 2015 Mar 28;(97):
pubmed: 25867928
Water Res. 2019 Apr 15;153:349-356
pubmed: 30743085
J Clin Virol. 2017 Sep;94:63-66
pubmed: 28759774
Transfus Apher Sci. 2016 Dec;55(3):271-274
pubmed: 27843081
J Gen Virol. 2017 Nov;98(11):2645-2646
pubmed: 29022866
Sci Total Environ. 2018 Oct 1;637-638:665-671
pubmed: 29758423
Viruses. 2019 May 17;11(5):
pubmed: 31108942
J Water Health. 2014 Sep;12(3):436-42
pubmed: 25252347
Viruses. 2019 Jul 05;11(7):
pubmed: 31284447
J Appl Microbiol. 2004;97(1):17-28
pubmed: 15186438
Int J Infect Dis. 2013 Apr;17(4):e228-33
pubmed: 23313154
Phys Ther. 2009 Sep;89(9):873-80
pubmed: 19723669
Arch Virol. 2010 Aug;155(8):1227-33
pubmed: 20532928
Microorganisms. 2019 Dec 26;8(1):
pubmed: 31888090
Appl Environ Microbiol. 2010 Feb;76(4):1120-4
pubmed: 20038700
Int J Hyg Environ Health. 2011 Nov;214(6):424-36
pubmed: 21920815
PLoS One. 2016 Nov 23;11(11):e0167105
pubmed: 27880820