A novel TEM grid sampler for airborne particles to measure the cell culture surface dose.
Aerosols
/ administration & dosage
Copper
/ chemistry
Culture Techniques
/ instrumentation
Equipment Design
Humans
Image Processing, Computer-Assisted
/ methods
Lung
/ cytology
Metal Nanoparticles
/ administration & dosage
Microscopy, Electron, Transmission
/ instrumentation
Particle Size
Particulate Matter
/ administration & dosage
Quartz Crystal Microbalance Techniques
Titanium
/ administration & dosage
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 05 2020
21 05 2020
Historique:
received:
24
02
2020
accepted:
04
05
2020
entrez:
23
5
2020
pubmed:
23
5
2020
medline:
15
1
2021
Statut:
epublish
Résumé
The applied surface dose is a key parameter for the measurement of toxic effects of airborne particles by air liquid interface exposure of human lung cells. Besides online measurement of the deposited particle mass by quartz crystal microbalance frequently other dose metrics such as particle size distribution, surface and agglomeration state are required. These particle properties and their spatial distribution can be determined by digital processing of micrographs obtained by transmission electron microscopy (TEM). Here, we report the development and characterization of a novel holder for film coated TEM copper grids, which allows for sampling under identical geometric and ambient conditions as in a cell culture chamber. The sample holder avoids artefacts by reliable grounding of the grids and improves handling of the grids to prevent damage of the sensitive film. This sample holder is applied during exposure experiments with titanium dioxide nanoparticles. The measured dose of 0.2 µg/cm² corresponds well to the mass loading signal of the quartz crystal microbalance. Additionally, the spatial distribution of particles on the sampling surface shows a good homogeneity of deposition. This novel sampling method allows verifying other dosimetry methods and gives additional information about particle properties and homogeneity of the dose.
Identifiants
pubmed: 32439902
doi: 10.1038/s41598-020-65427-w
pii: 10.1038/s41598-020-65427-w
pmc: PMC7242374
doi:
Substances chimiques
Aerosols
0
Particulate Matter
0
titanium dioxide
15FIX9V2JP
Copper
789U1901C5
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8401Références
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