Prebiotic effect of inulin-type fructans on faecal microbiota and short-chain fatty acids in type 2 diabetes: a randomised controlled trial.
16S rRNA sequencing
Faecal bacteria
Prebiotics
SCFA
Type 2 diabetes
Journal
European journal of nutrition
ISSN: 1436-6215
Titre abrégé: Eur J Nutr
Pays: Germany
ID NLM: 100888704
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
29
01
2020
accepted:
11
05
2020
pubmed:
23
5
2020
medline:
11
6
2021
entrez:
23
5
2020
Statut:
ppublish
Résumé
Compared to a healthy population, the gut microbiota in type 2 diabetes presents with several unfavourable features that may impair glucose regulation. The aim of this study was to evaluate the prebiotic effect of inulin-type fructans on the faecal microbiota and short-chain fatty acids (SCFA) in patients with type 2 diabetes. The study was a placebo controlled crossover study, where 25 patients (15 men) aged 41-71 years consumed 16 g of inulin-type fructans (a mixture of oligofructose and inulin) and 16-g placebo (maltodextrin) for 6 weeks in randomised order. A 4-week washout separated the 6 weeks treatments. The faecal microbiota was analysed by high-throughput 16S rRNA amplicon sequencing and SCFA in faeces were analysed using vacuum distillation followed by gas chromatography. Treatment with inulin-type fructans induced moderate changes in the faecal microbiota composition (1.5%, p = 0.045). A bifidogenic effect was most prominent, with highest positive effect on operational taxonomic units (OTUs) of Bifidobacterium adolescentis, followed by OTUs of Bacteroides. Significantly higher faecal concentrations of total SCFA, acetic acid and propionic acid were detected after prebiotic consumption compared to placebo. The prebiotic fibre had no effects on the concentration of butyric acid or on the overall microbial diversity. Six weeks supplementation with inulin-type fructans had a significant bifidogenic effect and induced increased concentrations of faecal SCFA, without changing faecal microbial diversity. Our findings suggest a moderate potential of inulin-type fructans to improve gut microbiota composition and to increase microbial fermentation in type 2 diabetes. The trial is registered at clinicaltrials.gov (NCT02569684).
Identifiants
pubmed: 32440730
doi: 10.1007/s00394-020-02282-5
pii: 10.1007/s00394-020-02282-5
pmc: PMC7501097
doi:
Substances chimiques
Fatty Acids, Volatile
0
Prebiotics
0
RNA, Ribosomal, 16S
0
Inulin
9005-80-5
Banques de données
ClinicalTrials.gov
['NCT02569684']
Types de publication
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Pagination
3325-3338Subventions
Organisme : The DAM Foundation, Norway
ID : 2013-2-267
Organisme : Diabetesforbundet
ID : 36660
Organisme : Mills DA
ID : 36660
Commentaires et corrections
Type : ErratumIn
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