Impact of Clinical Factors on the Intestinal Microbiome in Infants With Gastroschisis.

Cesarean Section Dysbiosis Female Gastrointestinal Microbiome Gastroschisis Humans Infant Pregnancy RNA, Ribosomal, 16S / genetics
gastroenterology microbiome pediatrics

Journal

JPEN. Journal of parenteral and enteral nutrition
ISSN: 1941-2444
Titre abrégé: JPEN J Parenter Enteral Nutr
Pays: United States
ID NLM: 7804134

Informations de publication

Date de publication:
05 2021
Historique:
revised: 04 05 2020
received: 09 10 2019
accepted: 11 05 2020
pubmed: 23 5 2020
medline: 9 7 2021
entrez: 23 5 2020
Statut: ppublish

Résumé

Infants with gastroschisis require operations and lengthy hospitalizations due to intestinal dysmotility. Dysbiosis may contribute to these problems. Little is known on the microbiome of gastroschisis infants. The purpose of this study was to investigate the fecal microbiome in gastroschisis infants. Microbiome profiling was performed by sequencing the V4 region of the 16S rRNA gene. The microbiome of gastroschisis infants was compared with the microbiome of healthy controls, and the effects of mode of birth delivery, gestational age, antibiotic duration, and nutrition type on microbial composition and diversity were investigated. The microbiome of gastroschisis infants (n = 13) was less diverse (Chao1, P < .001), lacked Bifidobacterium (P = .001), and had increased Staphylococcus (P = .007) compared with controls (n = 83). Mode of delivery (R The microbiome of gastroschisis infants is dysbiotic, and mode of birth delivery, antibiotic duration, and gestational age appear to contribute to microbial variation.

Sections du résumé

BACKGROUND
Infants with gastroschisis require operations and lengthy hospitalizations due to intestinal dysmotility. Dysbiosis may contribute to these problems. Little is known on the microbiome of gastroschisis infants.
METHODS
The purpose of this study was to investigate the fecal microbiome in gastroschisis infants. Microbiome profiling was performed by sequencing the V4 region of the 16S rRNA gene. The microbiome of gastroschisis infants was compared with the microbiome of healthy controls, and the effects of mode of birth delivery, gestational age, antibiotic duration, and nutrition type on microbial composition and diversity were investigated.
RESULTS
The microbiome of gastroschisis infants (n = 13) was less diverse (Chao1, P < .001), lacked Bifidobacterium (P = .001), and had increased Staphylococcus (P = .007) compared with controls (n = 83). Mode of delivery (R
CONCLUSION
The microbiome of gastroschisis infants is dysbiotic, and mode of birth delivery, antibiotic duration, and gestational age appear to contribute to microbial variation.

Identifiants

DOI: 10.1002/jpen.1926 PMID: 32441784 PMC: PMC9197548
pubmed: 32441784
doi: 10.1002/jpen.1926
pmc: PMC9197548
mid: NIHMS1804927
doi:

Substances chimiques

RNA, Ribosomal, 16S 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

818-825

Subventions

Organisme : NCATS NIH HHS
ID : KL2 TR000122
Pays : United States
Organisme : NCATS NIH HHS
ID : KL2 TR001882
Pays : United States
Organisme : NIH/NCATS
ID : KL2TR000122

Informations de copyright

© 2020 American Society for Parenteral and Enteral Nutrition.

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Auteurs

Allison J Wu (AJ)

Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Department of Pediatrics, Division of Gastroenterology, Harvard Medical School, Boston Children's Hospital, Boston, Massachusetts, USA.
ORCID: 0000-0002-3162-5439

David J Lee (DJ)

Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.

Fan Li (F)

Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.

Nicole H Tobin (NH)

Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.

Grace M Aldrovandi (GM)

Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.

Stephen B Shew (SB)

Department of Surgery, Division of Pediatric Surgery, Stanford University School of Medicine, Palo Alto, California, USA.

Kara L Calkins (KL)

Department of Pediatrics, Neonatal Research Center of the UCLA of Children's Discovery and Innovation Institute, David Geffen School of Medicine UCLA and UCLA Mattel Children's Hospital, Los Angeles, California, USA.
ORCID: 0000-0001-6658-7981

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Classifications MeSH

questionsmedicales.fr › Procédures de chirurgie opératoire › Procédures de chirurgie obstétrique › Accouchement (procédure) › Césarienne › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Dysbiose › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › États, signes et symptômes pathologiques › États anatomopathologiques › Hernie › Hernie abdominale › Laparoschisis › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › Personnes › Tranches d'âge › Nourrisson › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › Impact of Clinical Factors on the Intestinal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Impact of Clinical Factors on the Intestinal...