Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity.
Allergens
/ immunology
Animals
Antigens, Surface
/ immunology
Ceratopogonidae
/ immunology
Cytokines
/ metabolism
Horse Diseases
/ immunology
Horses
Hypersensitivity, Immediate
/ veterinary
Immunoglobulin E
/ blood
Insect Bites and Stings
/ immunology
Leukocytes, Mononuclear
/ cytology
Receptors, IgE
/ immunology
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
20
03
2020
accepted:
07
05
2020
entrez:
23
5
2020
pubmed:
23
5
2020
medline:
10
9
2020
Statut:
epublish
Résumé
Human IgE-binding monocytes are identified as allergic disease mediators, but it is unknown whether IgE-binding monocytes promote or prevent an allergic response. We identified IgE-binding monocytes in equine peripheral blood as IgE+/MHCIIhigh/CD14low cells that bind IgE through an FcεRI αɣ variant. IgE-binding monocytes were analyzed monthly in Culicoides hypersensitive horses and nonallergic horses living together with natural exposure to Culicoides midges. The phenotype and frequency of IgE-binding monocytes remained consistent in all horses regardless of Culicoides exposure. All horses upregulated IgE-binding monocyte CD16 expression following initial Culicoides exposure. Serum total IgE concentration and monocyte surface IgE densities were positively correlated in all horses. We also demonstrated that IgE-binding monocytes produce IL-10, but not IL-4, IL-17A, or IFN-γ, following IgE crosslinking. In conclusion, we have characterized horse IgE-binding monocytes for the first time and further studies of these cells may provide important connections between regulation and cellular mechanisms of IgE-mediated diseases.
Identifiants
pubmed: 32442209
doi: 10.1371/journal.pone.0233537
pii: PONE-D-20-08089
pmc: PMC7244122
doi:
Substances chimiques
Allergens
0
Antigens, Surface
0
Cytokines
0
Receptors, IgE
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0233537Subventions
Organisme : NCRR NIH HHS
ID : S10 RR025502
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Equine Vet J. 1988 Nov;20(6):426-9
pubmed: 3215168
Vet Immunol Immunopathol. 2013 Jun 15;153(3-4):227-39
pubmed: 23561552
J Allergy Clin Immunol. 2004 May;113(5):949-57
pubmed: 15131579
J Immunol. 1996 Jul 15;157(2):607-16
pubmed: 8752908
Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):63-80
pubmed: 17707518
J Immunol. 2001 Jul 15;167(2):797-804
pubmed: 11441085
Allergy. 2011 May;66(5):605-11
pubmed: 21241315
Med Vet Entomol. 1990 Oct;4(4):375-81
pubmed: 2133005
Clin Exp Allergy. 2006 Nov;36(11):1436-45
pubmed: 17083354
Nature. 1999 Nov 25;402(6760 Suppl):B24-30
pubmed: 10586892
Vet Rec. 1998 Jan 17;142(3):66-7
pubmed: 9481844
J Immunol. 1995 Jun 15;154(12):6285-90
pubmed: 7759866
Cell. 1996 Jun 28;85(7):985-95
pubmed: 8674126
J Clin Invest. 1997 Mar 1;99(5):915-25
pubmed: 9062349
Vet Dermatol. 2019 Dec;30(6):536-e163
pubmed: 31441172
Vet Immunol Immunopathol. 2012 Jun 30;147(3-4):113-26
pubmed: 22575371
J Allergy Clin Immunol. 2007 Feb;119(2):464-71
pubmed: 17291860
Vet Immunol Immunopathol. 1994 Jul;42(1):3-60
pubmed: 7975180
Front Immunol. 2015 Aug 17;6:423
pubmed: 26347746
Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):65-75
pubmed: 18082270
Vet Rec. 2003 Jun 14;152(24):748-51
pubmed: 12833935
Dev Comp Immunol. 2010 Apr;34(4):436-44
pubmed: 19995577
Vet Immunol Immunopathol. 2006 Apr 15;110(3-4):269-78
pubmed: 16343646
Acta Vet Scand. 1988;29(3-4):311-4
pubmed: 3256230
J Allergy Clin Immunol. 2013 Feb;131(2):491-500.e1-5
pubmed: 23374271
Vet Res. 2006 Nov-Dec;37(6):813-25
pubmed: 16973120
Nature. 1993 Sep 23;365(6444):340-3
pubmed: 7690905
Br Med Bull. 2000;56(4):936-55
pubmed: 11359630
J Immunol. 2008 May 1;180(9):5771-7
pubmed: 18424693
Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):57-64
pubmed: 18061278
Immunogenetics. 2003 May;55(2):122-5
pubmed: 12709836
Vet Immunol Immunopathol. 2010 Sep 15;137(1-2):76-83
pubmed: 20537727
J Allergy Clin Immunol. 1999 Aug;104(2 Pt 1):492-8
pubmed: 10452777
J Immunol. 2004 Sep 1;173(5):3215-22
pubmed: 15322183
Vet Immunol Immunopathol. 2012 Jul 15;148(1-2):139-44
pubmed: 21700344
Am J Respir Crit Care Med. 1998 Dec;158(6):1958-62
pubmed: 9847292
Immunol Lett. 1995 Jul-Aug;47(1-2):127-32
pubmed: 8537089
Vet Immunol Immunopathol. 2012 Apr 15;146(2):135-42
pubmed: 22424928
Vet Res Commun. 1983 Jan;6(1):13-23
pubmed: 6868343
Vet Immunol Immunopathol. 2010 Jan 15;133(1):40-50
pubmed: 19640590
Vet Immunol Immunopathol. 2008 Dec 15;126(3-4):302-8
pubmed: 18926574
Vet Immunol Immunopathol. 2010 Nov 15;138(1-2):149-53
pubmed: 20674042
Immunity. 2013 May 23;38(5):906-17
pubmed: 23643722
Vet Immunol Immunopathol. 2003 Mar 20;92(1-2):45-60
pubmed: 12628763
J Allergy Clin Immunol. 2019 Apr;143(4):1643-1646.e6
pubmed: 30639345
J Immunol. 2015 Feb 1;194(3):887-97
pubmed: 25527785
J Allergy Clin Immunol. 1997 May;99(5):699-706
pubmed: 9155838
Immunobiology. 2007;212(6):499-503
pubmed: 17544834
Vet Immunol Immunopathol. 2012 Apr 15;146(2):125-34
pubmed: 22405681
Equine Vet J. 2001 Nov;33(7):699-706
pubmed: 11770993
Vet Immunol Immunopathol. 2009 Nov 15;132(1):53-61
pubmed: 19836083
J Med Entomol. 1993 Jul;30(4):657-63
pubmed: 8360890
Vet Immunol Immunopathol. 2013 Jun 15;153(3-4):187-93
pubmed: 23541920
J Allergy Clin Immunol. 2000 Sep;106(3):514-20
pubmed: 10984372
Br J Dermatol. 2014 Jul;170 Suppl 1:2-6
pubmed: 24720512
Nat Med. 2012 May 04;18(5):693-704
pubmed: 22561833
Immunity. 2018 Oct 16;49(4):595-613
pubmed: 30332628
Acta Vet Scand. 2006 May 26;48:3
pubmed: 16987399
J Exp Med. 1994 Feb 1;179(2):745-50
pubmed: 8294882
Cell Mol Life Sci. 2015 Jun;72(12):2349-60
pubmed: 25715742
J Allergy Clin Immunol. 2013 Oct;132(4):789-801; quiz 788
pubmed: 24075190
Vet Immunol Immunopathol. 2011 Feb 15;139(2-4):200-9
pubmed: 21071100
J Allergy Clin Immunol. 2004 Sep;114(3):527-30
pubmed: 15356552
Vet Immunol Immunopathol. 2009 Feb 15;127(3-4):242-8
pubmed: 19027964
Immunogenetics. 2000 Aug;51(10):878-81
pubmed: 10970105