A novel method for near-infrared fluorescence imaging of the urethra during perineal and transanal surgery: demonstration in a cadaveric model.

Fluorescene imaging colorectal cancer fluorescence imaging guided surgery near-infrared transanal surgery urethra injury

Journal

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland
ISSN: 1463-1318
Titre abrégé: Colorectal Dis
Pays: England
ID NLM: 100883611

Informations de publication

Date de publication:
11 2020
Historique:
received: 20 04 2020
accepted: 04 05 2020
pubmed: 23 5 2020
medline: 19 8 2021
entrez: 23 5 2020
Statut: ppublish

Résumé

Transanal total mesorectal excision is a promising novel sphincter-saving procedure for low rectal cancer. However, the transanal bottom-up dissection is associated with increased rates of iatrogenic urethral injuries. Near-infrared fluorescence (NIRF) imaging, given its deeper tissue penetration, has been explored in a limited number of studies for enhanced intra-operative urethral visualization. In this study, we explored the feasibility of a novel, ultrabright, biocompatible fluorescent polymer to coat urinary catheters for the purpose of intra-operative urethral visualization. In an ex vivo experiment, using a near-infrared laparoscope, the fluorescent signal of a coated catheter (near-infrared coating of equipment, NICE) was qualitatively and quantitatively compared to the signal of indocyanine green (ICG)/Instillagel® mixtures and ICG-filled catheters at several concentrations. Also, in three male human torsos, using fluorescent urinary catheters, NIRF-guided perineal dissections and a transanal total mesorectal excision were performed. Intra-operative NIRF-based urethral visualization was performed systematically. During the qualitative and quantitative fluorescence signal assessment, NICE-coated catheters were clearly superior to the ICG-based solutions. In the cadaveric experiments, enhanced urethral visualization was possible even at early stages of dissection, when the organ was covered by several tissue layers. NICE-coated catheters represent a promising potential to allow for NIRF-based intra-operative urethral visualization.

Identifiants

pubmed: 32443182
doi: 10.1111/codi.15156
doi:

Substances chimiques

Indocyanine Green IX6J1063HV

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1749-1753

Subventions

Organisme : Fondation ARC pour la Recherche sur le Cancer
Organisme : SATT Connectus
ID : NICE

Informations de copyright

Colorectal Disease © 2020 The Association of Coloproctology of Great Britain and Ireland.

Références

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Auteurs

M Barberio (M)

IHU-Strasbourg, Institute of Image-Guided Surgery, Strasbourg, France.

M Al-Taher (M)

IHU-Strasbourg, Institute of Image-Guided Surgery, Strasbourg, France.

A Forgione (A)

IHU-Strasbourg, Institute of Image-Guided Surgery, Strasbourg, France.

A Hoskere Ashoka (A)

Laboratoire de Bio-imagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Strasbourg, France.

E Felli (E)

IHU-Strasbourg, Institute of Image-Guided Surgery, Strasbourg, France.
Institute of Physiology, EA3072 Mitochondria Respiration and Oxidative Stress, University of Strasbourg, Strasbourg, France.

V Agnus (V)

IHU-Strasbourg, Institute of Image-Guided Surgery, Strasbourg, France.

J Marescaux (J)

IRCAD, Research Institute against Digestive Cancer, Strasbourg, France.

A Klymchenko (A)

Laboratoire de Bio-imagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Strasbourg, France.

M Diana (M)

Institute of Physiology, EA3072 Mitochondria Respiration and Oxidative Stress, University of Strasbourg, Strasbourg, France.
IRCAD, Research Institute against Digestive Cancer, Strasbourg, France.
ICUBE Laboratory, Photonics Instrumentation for Health, Strasbourg, France.

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