A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping.
Animals
Betacoronavirus
/ genetics
COVID-19
Catalytic Domain
Coronavirus Infections
/ virology
DNA-Directed RNA Polymerases
/ metabolism
Exoribonucleases
/ chemistry
Genome, Viral
/ genetics
Humans
Methyltransferases
/ chemistry
Pandemics
Pneumonia, Viral
/ virology
Protein Conformation, alpha-Helical
RNA Helicases
/ chemistry
RNA, Messenger
/ metabolism
RNA, Viral
/ metabolism
SARS-CoV-2
Viral Nonstructural Proteins
/ chemistry
Viral Regulatory and Accessory Proteins
/ chemistry
Virus Replication
/ physiology
COVID19
RNA replication
SARS-CoV-2
infectious disease
protein structure
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
20 05 2020
20 05 2020
Historique:
received:
24
04
2020
revised:
14
05
2020
accepted:
19
05
2020
entrez:
24
5
2020
pubmed:
24
5
2020
medline:
9
6
2020
Statut:
epublish
Résumé
The current coronavirus disease-2019 (COVID-19) pandemic is due to the novel coronavirus SARS-CoV-2. The scientific community has mounted a strong response by accelerating research and innovation, and has quickly set the foundation for understanding the molecular determinants of the disease for the development of targeted therapeutic interventions. The replication of the viral genome within the infected cells is a key stage of the SARS-CoV-2 life cycle. It is a complex process involving the action of several viral and host proteins in order to perform RNA polymerization, proofreading and final capping. This review provides an update of the structural and functional data on the key actors of the replicatory machinery of SARS-CoV-2, to fill the gaps in the currently available structural data, which is mainly obtained through homology modeling. Moreover, learning from similar viruses, we collect data from the literature to reconstruct the pattern of interactions among the protein actors of the SARS-CoV-2 RNA polymerase machinery. Here, an important role is played by co-factors such as Nsp8 and Nsp10, not only as allosteric activators but also as molecular connectors that hold the entire machinery together to enhance the efficiency of RNA replication.
Identifiants
pubmed: 32443810
pii: cells9051267
doi: 10.3390/cells9051267
pmc: PMC7291026
pii:
doi:
Substances chimiques
NSP10 protein, SARS-CoV-2
0
RNA, Messenger
0
RNA, Viral
0
Viral Nonstructural Proteins
0
Viral Regulatory and Accessory Proteins
0
Methyltransferases
EC 2.1.1.-
Nsp13 protein, SARS-CoV
EC 2.1.1.-
Nsp16 protein, SARS virus
EC 2.1.1.-
nsp14 protein, SARS coronavirus
EC 2.1.1.56
DNA-Directed RNA Polymerases
EC 2.7.7.6
Exoribonucleases
EC 3.1.-
RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Références
Cell Res. 2020 Mar;30(3):269-271
pubmed: 32020029
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3484-9
pubmed: 19208801
Nat Commun. 2018 Apr 19;9(1):1558
pubmed: 29674694
Virus Res. 2013 Sep;176(1-2):45-52
pubmed: 23702198
Annu Rev Virol. 2015 Nov;2(1):265-88
pubmed: 26958916
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
J Virol. 2017 Feb 14;91(5):
pubmed: 28031370
Adv Exp Med Biol. 1998;440:313-8
pubmed: 9782298
Adv Virus Res. 1997;48:1-100
pubmed: 9233431
Lancet. 2020 Feb 22;395(10224):565-574
pubmed: 32007145
Biochem Biophys Res Commun. 2020 May 21;526(1):165-169
pubmed: 32201080
Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12694-9
pubmed: 15304651
J Virol. 2003 Oct;77(19):10515-27
pubmed: 12970436
Antimicrob Agents Chemother. 2012 Sep;56(9):4718-28
pubmed: 22733076
J Virol. 2004 Jun;78(11):5619-32
pubmed: 15140959
Cell. 1997 Jun 13;89(6):951-61
pubmed: 9200613
PLoS Pathog. 2017 Jun 26;13(6):e1006474
pubmed: 28651017
J Virol. 2018 Oct 29;92(22):
pubmed: 30135128
Virology. 1994 Aug 1;202(2):621-30
pubmed: 8030227
J Virol. 2005 Oct;79(20):12905-13
pubmed: 16188992
J Biol Chem. 2014 Sep 12;289(37):25783-96
pubmed: 25074927
Proc Natl Acad Sci U S A. 2017 May 23;114(21):E4251-E4260
pubmed: 28484023
Virology. 2018 Jan 1;513:75-84
pubmed: 29035788
Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3792-6
pubmed: 15007178
PLoS Pathog. 2010 May 06;6(5):e1000896
pubmed: 20463816
J Mol Biol. 2003 Aug 29;331(5):991-1004
pubmed: 12927536
J Med Chem. 2019 Mar 14;62(5):2333-2347
pubmed: 30721061
Structure. 2014 May 6;22(5):719-30
pubmed: 24704253
Sci Transl Med. 2017 Jun 28;9(396):
pubmed: 28659436
J Virol. 1999 Sep;73(9):7441-52
pubmed: 10438834
Int J Biol Sci. 2020 Mar 15;16(10):1724-1731
pubmed: 32226290
Viruses. 2014 Aug 07;6(8):2991-3018
pubmed: 25105276
Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E162-E171
pubmed: 29279395
J Virol. 2004 Jul;78(14):7833-8
pubmed: 15220459
Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11892-7
pubmed: 16882730
J Virol. 2016 May 12;90(11):5399-5414
pubmed: 27009949
Antiviral Res. 2019 Sep;169:104541
pubmed: 31233808
Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):596-601
pubmed: 26733676
J Virol. 2008 May;82(9):4471-9
pubmed: 18305050
Gene. 2003 Jan 2;302(1-2):129-38
pubmed: 12527203
RNA Biol. 2011 Mar-Apr;8(2):270-9
pubmed: 21593585
EMBO J. 1991 Jan;10(1):17-24
pubmed: 1989882
Int J Antimicrob Agents. 2020 Mar;55(3):105924
pubmed: 32081636
PLoS Biol. 2008 Sep 16;6(9):e226
pubmed: 18798692
Sci Rep. 2017 Mar 06;7:43395
pubmed: 28262699
Nat Struct Mol Biol. 2005 Nov;12(11):980-6
pubmed: 16228002
J Virol. 2006 Aug;80(16):7902-8
pubmed: 16873247
Trends Biochem Sci. 2003 Jun;28(6):329-35
pubmed: 12826405
Nat Immunol. 2011 Feb;12(2):137-43
pubmed: 21217758
J Virol. 2007 Oct;81(19):10280-91
pubmed: 17634238
J Biol Chem. 2008 Dec 5;283(49):34218-28
pubmed: 18940786
Nat Struct Mol Biol. 2009 Nov;16(11):1134-40
pubmed: 19838190
J Virol. 1998 Aug;72(8):6838-50
pubmed: 9658133
J Mol Biol. 1973 May 15;76(2):241-56
pubmed: 4737475
Nature. 2010 Nov 18;468(7322):452-6
pubmed: 21085181
Curr Opin Struct Biol. 2002 Dec;12(6):783-93
pubmed: 12504684
Curr Top Microbiol Immunol. 2005;287:57-94
pubmed: 15609509
J Virol. 2007 Nov;81(22):12135-44
pubmed: 17804504
Virology. 2005 May 10;335(2):165-76
pubmed: 15840516
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5108-13
pubmed: 16549795
N Engl J Med. 2020 Mar 5;382(10):929-936
pubmed: 32004427
Viruses. 2019 Apr 04;11(4):
pubmed: 30987343
Nucleic Acids Res. 2015 Sep 30;43(17):8416-34
pubmed: 26304538
Biochemistry. 2019 Jan 22;58(3):166-170
pubmed: 30406995
Lancet. 2020 Feb 29;395(10225):689-697
pubmed: 32014114
Nat Commun. 2019 May 28;10(1):2342
pubmed: 31138817
EMBO J. 2006 Oct 18;25(20):4933-42
pubmed: 17024178
mBio. 2013 Aug 13;4(4):
pubmed: 23943763
Structure. 2004 Feb;12(2):341-53
pubmed: 14962394
J Virol. 2013 Jan;87(1):177-86
pubmed: 23055566
Antiviral Res. 2014 Mar;103:39-50
pubmed: 24418573
J Biol Chem. 2000 Feb 25;275(8):5329-36
pubmed: 10681506
mBio. 2018 Mar 6;9(2):
pubmed: 29511076
Nature. 2020 Apr 30;:
pubmed: 32353859
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6771-6776
pubmed: 32054787
RNA Biol. 2014;11(12):1597-607
pubmed: 25626080
Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22505-10
pubmed: 21148772
Virus Res. 2006 Apr;117(1):17-37
pubmed: 16503362
Nucleic Acids Res. 2019 Jul 9;47(12):6538-6550
pubmed: 31131400
J Virol. 2002 May;76(10):4987-99
pubmed: 11967315
J Biol Chem. 2020 Apr 10;295(15):4773-4779
pubmed: 32094225
FEBS Lett. 2006 Jul 24;580(17):4143-9
pubmed: 16828088
J Biol Chem. 2008 Feb 8;283(6):3655-64
pubmed: 18045871
Front Microbiol. 2019 Aug 07;10:1813
pubmed: 31440227
J Virol. 2000 Jun;74(11):5213-23
pubmed: 10799597
mBio. 2017 Jan 31;8(1):
pubmed: 28143984
Antimicrob Agents Chemother. 2014 Aug;58(8):4894-8
pubmed: 24841268
PLoS Pathog. 2018 Feb 9;14(2):e1006889
pubmed: 29425244
Virus Res. 2015 Apr 16;202:12-32
pubmed: 25497126
Cell Rep. 2017 Oct 24;21(4):1063-1076
pubmed: 29069588
Curr Med Chem. 2015;22(14):1745-56
pubmed: 25882544
mSphere. 2016 Sep 07;1(5):
pubmed: 27631026
Protein Sci. 2020 Apr 17;:
pubmed: 32304108
Science. 2020 May 15;368(6492):779-782
pubmed: 32277040
Chembiochem. 2020 Mar 2;21(5):730-738
pubmed: 32022370
Nat Commun. 2020 Jan 10;11(1):222
pubmed: 31924756
J Virol. 2006 Aug;80(16):7894-901
pubmed: 16873246
Nature. 2016 Mar 17;531(7594):381-5
pubmed: 26934220
J Virol. 2011 May;85(10):4963-73
pubmed: 21389138
J Virol. 2005 Jan;79(2):696-704
pubmed: 15613297
Curr Opin Struct Biol. 2007 Jun;17(3):316-24
pubmed: 17574830
Cell Host Microbe. 2020 Mar 11;27(3):325-328
pubmed: 32035028
Nat Rev Microbiol. 2011 Dec 05;10(1):51-65
pubmed: 22138959
PLoS Pathog. 2011 May;7(5):e1002059
pubmed: 21637813
PLoS Pathog. 2013 Aug;9(8):e1003565
pubmed: 23966862
Proc Natl Acad Sci U S A. 2016 May 10;113(19):5388-93
pubmed: 27118832
Nucleic Acids Res. 2014 Mar;42(5):3464-77
pubmed: 24369429
J Virol. 2007 Jan;81(1):20-9
pubmed: 16928755
J Virol. 2008 Dec;82(24):12346-55
pubmed: 18829745
PLoS Pathog. 2011 Oct;7(10):e1002294
pubmed: 22022266
J Virol. 1980 Jan;33(1):449-62
pubmed: 6245243
PLoS One. 2012;7(5):e36521
pubmed: 22615777
Cell Mol Immunol. 2020 Feb 11;:
pubmed: 32047258
Virology. 2015 May;479-480:66-74
pubmed: 25682435
Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):E3900-9
pubmed: 25197083
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
J Virol. 2004 Nov;78(22):12218-24
pubmed: 15507608
Science. 2020 May 1;:
pubmed: 32358203
Nat Rev Immunol. 2013 Jan;13(1):46-57
pubmed: 23237964
Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):9436-41
pubmed: 26159422
PLoS One. 2007 May 23;2(5):e459
pubmed: 17520018
Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9372-7
pubmed: 22635272
J Mol Biol. 2010 Oct 1;402(4):619-28
pubmed: 20709084
J Virol. 2013 Jun;87(11):6296-305
pubmed: 23536667
J Biomol Struct Dyn. 2020 May;38(8):2455-2467
pubmed: 31299874
Annu Rev Pharmacol Toxicol. 2017 Jan 6;57:329-348
pubmed: 27959624
Adv Virus Res. 2005;64:165-230
pubmed: 16139595
Virus Res. 2014 Dec 19;194:90-9
pubmed: 25451065
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508