Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.
DNA curtain
SMC complexes
chromosome organization
condensin
crosslinking mass spectroscopy
electron microscopy
loop extrusion
single molecule
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
02 07 2020
02 07 2020
Historique:
received:
23
09
2019
revised:
08
02
2020
accepted:
22
04
2020
pubmed:
24
5
2020
medline:
25
8
2020
entrez:
24
5
2020
Statut:
ppublish
Résumé
Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells.
Identifiants
pubmed: 32445620
pii: S1097-2765(20)30268-9
doi: 10.1016/j.molcel.2020.04.026
pmc: PMC7335352
mid: NIHMS1598412
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Multiprotein Complexes
0
Nucleosomes
0
condensin complexes
0
Adenosine Triphosphate
8L70Q75FXE
DNA
9007-49-2
Adenosine Triphosphatases
EC 3.6.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
99-114.e9Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 200818/Z/16/Z
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R35 GM118026
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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