High subzero cryofixation: A technique for observing ice within tissues.

Animals Cryopreservation / methods Freezing Ice Rats Temperature Water
Cryofixation Freeze substitution High sub-zero organ preservation Ice recrystallization Isothermal freeze fixation Tissue fixation

Journal

Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252

Informations de publication

Date de publication:
08 2020
Historique:
received: 31 12 2019
revised: 20 05 2020
accepted: 21 05 2020
pubmed: 26 5 2020
medline: 16 2 2021
entrez: 26 5 2020
Statut: ppublish

Résumé

While various fixation techniques for observing ice within tissues stored at high sub-zero temperatures currently exist, these techniques require either different fixative solution compositions when assessing different storage temperatures or alteration of the sample temperature to enable alcohol-water substitution. Therefore, high-subzero cryofixation (HSC), was developed to facilitate fixation at any temperature above -80 °C without sample temperature alteration. Rat liver sections (1 cm

Identifiants

DOI: 10.1016/j.cryobiol.2020.05.008 PMID: 32450134
pubmed: 32450134
pii: S0011-2240(19)30649-2
doi: 10.1016/j.cryobiol.2020.05.008
pii:
doi:

Substances chimiques

Ice 0
Water 059QF0KO0R

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

116-122

Subventions

Organisme : CIHR
ID : PJT 159847
Pays : Canada

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Larissa Lautner (L)

Department of Surgery, University of Alberta, Edmonton, AB, T6G 2B7, Canada.

Nishaka William (N)

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2R3, Canada.

Jason P Acker (JP)

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 2R3, Canada; Centre for Innovation, Canadian Blood Services, 8249 114th Street, Edmonton, AB, T6G 2R8, Canada. Electronic address: jacker@ualberta.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › High subzero cryofixation: A technique for...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Cryoconservation › High subzero cryofixation: A technique for...
questionsmedicales.fr › Phénomènes chimiques › Transition de phase › Congélation › High subzero cryofixation: A technique for...
questionsmedicales.fr › Environnement et santé publique › Environnement › Glace › High subzero cryofixation: A technique for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › High subzero cryofixation: A technique for...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › High subzero cryofixation: A technique for...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Eau › High subzero cryofixation: A technique for...