BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.
Circadian Clock
Molecular Mechanism of Gene Regulation
Nucleolus
Ribosomal RNA
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
26 Jun 2020
26 Jun 2020
Historique:
received:
14
02
2020
revised:
30
03
2020
accepted:
05
05
2020
pubmed:
26
5
2020
medline:
26
5
2020
entrez:
26
5
2020
Statut:
ppublish
Résumé
The transcription factor BMAL1 is a core element of the circadian clock that contributes to cyclic control of genes transcribed by RNA polymerase II. By using biochemical cellular fractionation and immunofluorescence analyses we reveal a previously uncharacterized nucleolar localization for BMAL1. We used an unbiased approach to determine the BMAL1 interactome by mass spectrometry and identified NOP58 as a prominent nucleolar interactor. NOP58, a core component of the box C/D small nucleolar ribonucleoprotein complex, associates with Snord118 to control specific pre-ribosomal RNA (pre-rRNA) processing steps. These results suggest a non-canonical role of BMAL1 in ribosomal RNA regulation. Indeed, we show that BMAL1 controls NOP58-associated Snord118 nucleolar levels and cleavage of unique pre-rRNA intermediates. Our findings identify an unsuspected function of BMAL1 in the nucleolus that appears distinct from its canonical role in the circadian clock system.
Identifiants
pubmed: 32450515
pii: S2589-0042(20)30336-9
doi: 10.1016/j.isci.2020.101151
pmc: PMC7256328
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101151Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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