Ocular hypertension suppresses homeostatic gene expression in optic nerve head microglia of DBA/2 J mice.


Journal

Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876

Informations de publication

Date de publication:
25 05 2020
Historique:
received: 22 12 2019
accepted: 13 04 2020
entrez: 27 5 2020
pubmed: 27 5 2020
medline: 5 6 2021
Statut: epublish

Résumé

Glaucoma is the leading cause of irreversible vision loss. Ocular hypertension is a major risk factor for glaucoma and recent work has demonstrated critical early neuroinflammatory insults occur in the optic nerve head following ocular hypertension. Microglia and infiltrating monocytes are likely candidates to drive these neuroinflammatory insults. However, the exact molecular identity / transcriptomic profile of microglia following ocular hypertensive insults is unknown. To elucidate the molecular identity of microglia after long-term exposure to ocular hypertension, we used a mouse model of glaucoma (DBA/2 J). We performed RNA-sequencing of microglia mRNA from the optic nerve head at a time point following ocular hypertensive insults, but preceding detectable neurodegeneration (with microglia identified as being CD45

Identifiants

pubmed: 32450896
doi: 10.1186/s13041-020-00603-7
pii: 10.1186/s13041-020-00603-7
pmc: PMC7249412
doi:

Substances chimiques

RNA, Messenger 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

81

Subventions

Organisme : NEI NIH HHS
ID : EY011721
Pays : United States
Organisme : Vetenskapsrådet
ID : 2018-02124
Pays : International

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Auteurs

James R Tribble (JR)

Department of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Stockholm, Sweden.

Jeffrey M Harder (JM)

The Howard Hughes Medical Institute, The Jackson Laboratory, Bar Harbor, ME, USA.

Pete A Williams (PA)

Department of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Stockholm, Sweden. pete.williams@ki.se.

Simon W M John (SWM)

The Howard Hughes Medical Institute, The Jackson Laboratory, Bar Harbor, ME, USA. sj2967@cumc.columbia.edu.
Department of Ophthalmology and Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA. sj2967@cumc.columbia.edu.

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Classifications MeSH