SCREENED: A Multistage Model of Thyroid Gland Function for Screening Endocrine-Disrupting Chemicals in a Biologically Sex-Specific Manner.
bioprinting
decellularization
endocrine disruptors
in vitro models
omics
organoids
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 May 2020
21 May 2020
Historique:
received:
16
03
2020
revised:
04
05
2020
accepted:
14
05
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Endocrine disruptors (EDs) are chemicals that contribute to health problems by interfering with the physiological production and target effects of hormones, with proven impacts on a number of endocrine systems including the thyroid gland. Exposure to EDs has also been associated with impairment of the reproductive system and incidence in occurrence of obesity, type 2 diabetes, and cardiovascular diseases during ageing. SCREENED aims at developing in vitro assays based on rodent and human thyroid cells organized in three different three-dimensional (3D) constructs. Due to different levels of anatomical complexity, each of these constructs has the potential to increasingly mimic the structure and function of the native thyroid gland, ultimately achieving relevant features of its 3D organization including: 1) a 3D organoid based on stem cell-derived thyrocytes, 2) a 3D organoid based on a decellularized thyroid lobe stromal matrix repopulated with stem cell-derived thyrocytes, and 3) a bioprinted organoid based on stem cell-derived thyrocytes able to mimic the spatial and geometrical features of a native thyroid gland. These 3D constructs will be hosted in a modular microbioreactor equipped with innovative sensing technology and enabling precise control of cell culture conditions. New superparamagnetic biocompatible and biomimetic particles will be used to produce "magnetic cells" to support precise spatiotemporal homing of the cells in the 3D decellularized and bioprinted constructs. Finally, these 3D constructs will be used to screen the effect of EDs on the thyroid function in a unique biological sex-specific manner. Their performance will be assessed individually, in comparison with each other, and against in vivo studies. The resulting 3D assays are expected to yield responses to low doses of different EDs, with sensitivity and specificity higher than that of classical 2D in vitro assays and animal models. Supporting the "Adverse Outcome Pathway" concept, proteogenomic analysis and biological computational modelling of the underlying mode of action of the tested EDs will be pursued to gain a mechanistic understanding of the chain of events from exposure to adverse toxic effects on thyroid function. For future uptake, SCREENED will engage discussion with relevant stakeholder groups, including regulatory bodies and industry, to ensure that the assays will fit with purposes of ED safety assessment. In this project review, we will briefly discuss the current state of the art in cellular assays of EDs and how our project aims at further advancing the field of cellular assays for EDs interfering with the thyroid gland.
Identifiants
pubmed: 32455722
pii: ijms21103648
doi: 10.3390/ijms21103648
pmc: PMC7279272
pii:
doi:
Substances chimiques
Endocrine Disruptors
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Horizon 2020 Framework Programme
ID : 825745
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Endocrinology. 2018 Apr 1;159(4):1910-1921
pubmed: 29522169
EuPA Open Proteom. 2017 Sep 13;17:1-6
pubmed: 29900122
Nat Biomed Eng. 2017 Dec;1(12):939-956
pubmed: 31015708
Inorg Chem. 2017 Apr 17;56(8):4447-4459
pubmed: 28379709
Acta Biomater. 2012 Feb;8(2):843-51
pubmed: 22005331
Eur Thyroid J. 2017 Jul;6(4):171-177
pubmed: 28868257
Nat Methods. 2009 Oct;6(10):711-13
pubmed: 19953680
J Endocrinol. 2007 Jan;192(1):121-30
pubmed: 17210749
Nat Genet. 2008 Dec;40(12):1413-5
pubmed: 18978789
Tumour Biol. 2016 Jul;37(7):8923-30
pubmed: 26753958
Int J Mol Sci. 2019 Sep 26;20(19):
pubmed: 31561483
Int J Mol Sci. 2013 Jan 09;14(1):1164-78
pubmed: 23303277
J Mol Endocrinol. 2011 Feb;46(1):R33-42
pubmed: 21322126
Mol Cell Proteomics. 2014 Feb;13(2):397-406
pubmed: 24309898
J Cell Biochem. 2004 Aug 15;92(6):1246-56
pubmed: 15258907
Mol Cell Endocrinol. 2012 May 22;355(2):240-8
pubmed: 21939731
Cell Stem Cell. 2015 Nov 5;17(5):527-42
pubmed: 26593959
Sci Rep. 2016 Oct 03;6:34410
pubmed: 27694985
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3394-9
pubmed: 20133684
Environ Res. 2012 Jan;112:204-11
pubmed: 22030286
J Appl Toxicol. 2015 Sep;35(9):992-8
pubmed: 25644787
Oncol Rep. 2015 Apr;33(4):1837-43
pubmed: 25634491
Nanomedicine (Lond). 2012 Sep;7(9):1425-42
pubmed: 22994959
Mol Syst Biol. 2019 Feb 18;15(2):e8503
pubmed: 30777892
Nano Lett. 2012 Aug 8;12(8):4131-9
pubmed: 22769232
J Clin Endocrinol Metab. 1983 Feb;56(2):260-3
pubmed: 6822636
Thyroid. 2015 Apr;25(4):455-61
pubmed: 25585054
Environ Toxicol Chem. 2016 Apr;35(4):788-93
pubmed: 25982118
Endocr Rev. 2015 Dec;36(6):593-602
pubmed: 26414233
Toxicol Sci. 2012 Dec;130(2):217-28
pubmed: 22790972
Thyroid. 2006 Jun;16(6):537-44
pubmed: 16839255
Exp Cell Res. 2015 Nov 1;338(2):127-35
pubmed: 26384551
Proc Natl Acad Sci U S A. 2017 Jun 20;114(25):6581-6586
pubmed: 28607076
Proteomics. 2012 Apr;12(8):1093-110
pubmed: 22577011
Mol Cell Endocrinol. 2005 Dec 1;244(1-2):63-71
pubmed: 16289305
Cell Rep. 2014 Aug 21;8(4):923-32
pubmed: 25127145
J Tissue Eng Regen Med. 2012 Oct;6(9):696-701
pubmed: 21932277
Biosens Bioelectron. 2013 Jul 15;45:6-12
pubmed: 23454336
Mol Cell Endocrinol. 2018 Sep 5;472:68-79
pubmed: 29183805
J Cell Mol Med. 2015 Aug;19(8):2032-5
pubmed: 26041641
J Med Invest. 2003 Aug;50(3-4):192-8
pubmed: 13678390
Clin Chem. 2010 Feb;56(2):186-93
pubmed: 19959625
Aquat Toxicol. 2015 Mar;160:188-96
pubmed: 25646720
Sci Rep. 2016 Dec 01;6:38131
pubmed: 27905518
Adv Mater. 2011 Mar 18;23(11):1311-29
pubmed: 21400590
Genome Res. 2008 Apr;18(4):610-21
pubmed: 18285502
Int J Mol Sci. 2017 Dec 01;18(12):
pubmed: 29194390
Ann Anat. 2011 Oct 20;193(5):381-94
pubmed: 21803554
Toxicol Sci. 2013 May;133(1):67-78
pubmed: 23377618
J Environ Monit. 2012 Aug;14(8):2121-6
pubmed: 22699773
J Proteome Res. 2017 Aug 4;16(8):2680-2691
pubmed: 28678506
Endocr J. 2001 Aug;48(4):407-25
pubmed: 11603563
Curr Med Chem. 2019;26(10):1874-1890
pubmed: 28260501
Curr Opin Genet Dev. 2017 Oct;46:149-155
pubmed: 28806595
Oncotarget. 2017 Feb 28;8(9):14374-14394
pubmed: 28086232
Curr Opin Biotechnol. 2001 Apr;12(2):180-7
pubmed: 11287235
Biomaterials. 2006 Dec;27(36):6032-42
pubmed: 16884768
Thyroid. 2016 Aug;26(8):1131-40
pubmed: 27296473
Methods Mol Biol. 2017;1597:85-95
pubmed: 28361312
Environ Toxicol Pharmacol. 2016 Dec;48:168-174
pubmed: 27794274
Front Physiol. 2017 Mar 08;8:123
pubmed: 28337147
Endocr Rev. 2003 Feb;24(1):48-77
pubmed: 12588808
Biomaterials. 2015 Jan;38:108
pubmed: 25499931
J Endocrinol. 2000 Oct;167(1):125-35
pubmed: 11018760
Chem Rev. 2014 Jun 11;114(11):5641-94
pubmed: 24779750
J Endocrinol. 2008 Sep;198(3):471-87
pubmed: 18550786
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3455-9
pubmed: 6106191
Cell. 2016 Apr 21;165(3):535-50
pubmed: 27104977
Indian J Endocrinol Metab. 2013 Mar;17(2):219-27
pubmed: 23776892
Endocrinology. 2007 Sep;148(9):4251-8
pubmed: 17584961
Br J Cancer. 1989 Dec;60(6):897-903
pubmed: 2557880
Nat Biotechnol. 2014 Sep;32(9):926-32
pubmed: 25150839
Environ Sci Technol. 2011 Sep 15;45(18):7906-14
pubmed: 21809831
Mol Cell Proteomics. 2013 Jul;12(7):1965-79
pubmed: 23579186
High Throughput. 2018 Oct 26;7(4):
pubmed: 30373182
Cell Stem Cell. 2016 Jan 7;18(1):25-38
pubmed: 26748754
J Toxicol Environ Health A. 2011;74(14):917-26
pubmed: 21623536
Horm Metab Res. 1987 Aug;19(8):391-2
pubmed: 3311957
Sci Rep. 2016 Mar 30;6:23660
pubmed: 27025787
J Toxicol Environ Health A. 2014;77(9-11):516-34
pubmed: 24754389
Endocr Rev. 2001 Oct;22(5):631-56
pubmed: 11588145
Toxicol Sci. 2006 Feb;89(2):408-14
pubmed: 16291828
Cell. 2016 May 19;165(5):1238-1254
pubmed: 27118425
Sci Total Environ. 2018 Jul 1;628-629:1489-1496
pubmed: 30045567
Development. 2017 Mar 15;144(6):938-941
pubmed: 28292837
Endocr Rev. 2004 Oct;25(5):722-46
pubmed: 15466939
FEBS Lett. 2005 Mar 28;579(9):1900-3
pubmed: 15792793
Reproduction. 2015 Nov;150(5):R149-57
pubmed: 26416010
Nat Commun. 2014 May 14;5:3846
pubmed: 24828763
Environ Sci Technol. 2008 Mar 1;42(5):1773-9
pubmed: 18441834
Science. 2008 Feb 15;319(5865):906-7
pubmed: 18276874
Paediatr Perinat Epidemiol. 2018 May;32(3):225-234
pubmed: 29517803
Int J Mol Sci. 2017 Mar 03;18(3):
pubmed: 28273809
PLoS One. 2016 Mar 17;11(3):e0151192
pubmed: 26985823
Curr Biol. 2006 Jan 24;16(2):166-72
pubmed: 16431368
Am J Respir Cell Mol Biol. 2019 Aug;61(2):143-149
pubmed: 30874442
Endokrinologie. 1981 Jul;77(3):297-302
pubmed: 7274159
Nat Rev Mol Cell Biol. 2014 Aug;15(8):509-24
pubmed: 25027649
J Proteome Res. 2009 Dec;8(12):5411
pubmed: 19863051
Toxicology. 2007 Jul 31;237(1-3):65-76
pubmed: 17553607
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
PLoS One. 2016 Mar 16;11(3):e0151618
pubmed: 26982218
Biofabrication. 2017 Aug 18;9(3):034105
pubmed: 28707625
Acta Anat (Basel). 1986;127(2):115-8
pubmed: 3788452
Nature. 2014 Sep 18;513(7518):382-7
pubmed: 25043054
Sci Rep. 2017 Jan 09;7:40153
pubmed: 28065941
Thyroid. 2015 Aug;25(8):954-61
pubmed: 26061389
Biomaterials. 2018 Feb;154:113-133
pubmed: 29120815
J Hum Genet. 2018 Feb;63(2):165-178
pubmed: 29192237
Mol Carcinog. 1999 Mar;24(3):153-9
pubmed: 10204799
ALTEX. 2018 Feb 23;35(3):306-352
pubmed: 29485663
Nature. 2012 Nov 1;491(7422):66-71
pubmed: 23051751
MOJ Proteom Bioinform. 2015;2(2):44-57
pubmed: 31360934
Mol Cell. 2013 Feb 21;49(4):583-90
pubmed: 23438854
J Thyroid Res. 2012;2012:815079
pubmed: 22518348
J Proteomics. 2018 Jan 6;170:151-166
pubmed: 28823580
Food Chem Toxicol. 2019 Sep;131:110552
pubmed: 31163220
Proteomics. 2016 Mar;16(6):907-14
pubmed: 26791339
Cell Stem Cell. 2015 Jun 4;16(6):588-90
pubmed: 26046758
BMC Genomics. 2015 Apr 14;16:295
pubmed: 25888192