In-Vitro-Generated Hypertrophic-Like Adipocytes Displaying
Adipocytes
/ metabolism
Adipose Tissue
/ pathology
Cell Differentiation
Cell Line
Cell Shape
Cell Size
Female
Glucose Transporter Type 4
/ metabolism
Humans
Hypertrophy
Lipid Droplets
/ metabolism
Male
Mesenchymal Stem Cells
/ metabolism
Middle Aged
Models, Biological
Obesity
/ metabolism
PPAR gamma
/ metabolism
Protein Isoforms
/ metabolism
PPARG isoforms
PPARG splicing
adipogenesis
dominant-negative isoform
hypertrophic adipocytes
hypertrophic obesity
in vitro adipocytes
insulin-resistance
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
21 05 2020
21 05 2020
Historique:
received:
30
04
2020
revised:
18
05
2020
accepted:
19
05
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
26
2
2021
Statut:
epublish
Résumé
Reduced neo-adipogenesis and dysfunctional lipid-overloaded adipocytes are hallmarks of hypertrophic obesity linked to insulin resistance. Identifying molecular features of hypertrophic adipocytes requires appropriate in vitro models. We describe the generation of a model of human hypertrophic-like adipocytes directly comparable to normal adipose cells and the pathologic evolution toward hypertrophic state. We generate in vitro hypertrophic cells from mature adipocytes, differentiated from human mesenchymal stem cells. Combining optical, confocal, and transmission electron microscopy with mRNA/protein quantification, we characterize this cellular model, confirming specific alterations also in subcutaneous adipose tissue. Specifically, we report the generation and morphological/molecular characterization of human normal and hypertrophic-like adipocytes. The latter displays altered morphology and unbalance between canonical and dominant negative (PPARGΔ5) transcripts of
Identifiants
pubmed: 32455814
pii: cells9051284
doi: 10.3390/cells9051284
pmc: PMC7290899
pii:
doi:
Substances chimiques
Glucose Transporter Type 4
0
PPAR gamma
0
Protein Isoforms
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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