Sonic Hedgehog and Triiodothyronine Pathway Interact in Mouse Embryonic Neural Stem Cells.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
23 May 2020
Historique:
received: 09 04 2020
revised: 13 05 2020
accepted: 19 05 2020
entrez: 28 5 2020
pubmed: 28 5 2020
medline: 17 2 2021
Statut: epublish

Résumé

Neural stem cells are fundamental to development of the central nervous system (CNS)-as well as its plasticity and regeneration-and represent a potential tool for neuro transplantation therapy and research. This study is focused on examination of the proliferation dynamic and fate of embryonic neural stem cells (eNSCs) under differentiating conditions. In this work, we analyzed eNSCs differentiating alone and in the presence of sonic hedgehog (SHH) or triiodothyronine (T3) which play an important role in the development of the CNS. We found that inhibition of the SHH pathway and activation of the T3 pathway increased cellular health and survival of differentiating eNSCs. In addition, T3 was able to increase the expression of the gene for the receptor smoothened (

Identifiants

pubmed: 32456161
pii: ijms21103672
doi: 10.3390/ijms21103672
pmc: PMC7279276
pii:
doi:

Substances chimiques

Hedgehog Proteins 0
Shh protein, mouse 0
Smo protein, mouse 0
Smoothened Receptor 0
Thyroid Hormone Receptors beta 0
Triiodothyronine 06LU7C9H1V
iodothyronine deiodinase type III EC 1.11.1.-
Iodide Peroxidase EC 1.11.1.8

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Ministry of Education Youth and Sports of the Czech Republic
ID : National Sustainability Program I (NPU I) Nr. LO1503
Organisme : Charles University Research Fund
ID : project number Q39
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : No. CZ.02.1.01/0.0/0.0/16_019/0000787 "Fighting INfectious Diseases"

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Auteurs

Pavel Ostasov (P)

Laboratory of Tumor Biology and Immunotherapy, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Plzen, Czech Republic.

Jan Tuma (J)

Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.
Laboratory of Neurodegenerative Disorders, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.

Pavel Pitule (P)

Laboratory of Tumor Biology and Immunotherapy, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Plzen, Czech Republic.

Jiri Moravec (J)

Laboratory of Proteomics, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.

Zbynek Houdek (Z)

Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.
Department of Biology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.

Frantisek Vozeh (F)

Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.
Laboratory of Neurodegenerative Disorders, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.

Milena Kralickova (M)

Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Plzen, Czech Republic.

Jan Cendelin (J)

Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.
Laboratory of Neurodegenerative Disorders, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00 Plzen, Czech Republic.

Vaclav Babuska (V)

Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Plzen, Czech Republic.

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Classifications MeSH