Analysis of the Level of Plasmid-Derived mRNA in the Presence of Residual Plasmid DNA by Two-Step Quantitative RT-PCR.
DNA contamination
endogenous genome-derived mRNA
genomic DNA
nonsense-tail PCR primer
nonsense-tail reverse transcription
overexpression experiments
plasmid DNA
plasmid-derived mRNA
Journal
Methods and protocols
ISSN: 2409-9279
Titre abrégé: Methods Protoc
Pays: Switzerland
ID NLM: 101720073
Informations de publication
Date de publication:
23 May 2020
23 May 2020
Historique:
received:
19
02
2020
revised:
19
05
2020
accepted:
21
05
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
28
5
2020
Statut:
epublish
Résumé
In transfection experiments with mammalian cells aiming to overexpress a specific protein, it is often necessary to correctly quantify the level of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteoblasts were transfected with a vector containing the complete
Identifiants
pubmed: 32456168
pii: mps3020040
doi: 10.3390/mps3020040
pmc: PMC7359704
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : This study was supported by internal performance-related resource allocation ("Leistungsorientierte Mittel") of the Justus Liebig University Giessen.
ID : no grant number
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