An evolutionarily conserved RNA structure in the functional core of the lincRNA Cyrano.
Cyrano
SHAPE
conservation
lincRNA
structure
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
27
04
2020
accepted:
18
05
2020
pubmed:
28
5
2020
medline:
6
10
2020
entrez:
28
5
2020
Statut:
ppublish
Résumé
The wide prevalence and regulated expression of long noncoding RNAs (lncRNAs) highlight their functional roles, but the molecular basis for their activities and structure-function relationships remains to be investigated, with few exceptions. Among the relatively few lncRNAs conserved over significant evolutionary distances is the long intergenic noncoding RNA (lincRNA) Cyrano (orthologous to human OIP5-AS1), which contains a region of 300 highly conserved nucleotides within tetrapods, which in turn contains a functional stretch of 26 nt of deep conservation. This region binds to and facilitates the degradation of the microRNA miR-7, a short ncRNA with multiple cellular functions, including modulation of oncogenic expression. We probed the secondary structure of Cyrano in vitro and in cells using chemical and enzymatic probing, and validated the results using comparative sequence analysis. At the center of the functional core of Cyrano is a cloverleaf structure maintained over the >400 million years of divergent evolution that separates fish and primates. This strikingly conserved motif provides interaction sites for several RNA-binding proteins and masks a conserved recognition site for miR-7. Conservation in this region strongly suggests that the function of Cyrano depends on the formation of this RNA structure, which could modulate the rate and efficiency of degradation of miR-7.
Identifiants
pubmed: 32457084
pii: rna.076117.120
doi: 10.1261/rna.076117.120
pmc: PMC7430676
doi:
Substances chimiques
MicroRNAs
0
RNA, Long Noncoding
0
RNA, Messenger
0
RNA, Untranslated
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1234-1246Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM103834
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121487
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM086304
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM126942
Pays : United States
Informations de copyright
© 2020 Jones et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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