Structure-Function Relationship of Inclusion Bodies of a Multimeric Protein.

active inclusion bodies amyloid content amyloid structure biological activity inclusion bodies

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2020
Historique:
received: 29 02 2020
accepted: 15 04 2020
entrez: 28 5 2020
pubmed: 28 5 2020
medline: 28 5 2020
Statut: epublish

Résumé

High level expression of recombinant proteins in bacteria often results in their aggregation into inclusion bodies. Formation of inclusion bodies poses a major bottleneck in high-throughput recovery of recombinant protein. These aggregates have amyloid-like nature and can retain biological activity. Here, effect of expression temperature on the quality of

Identifiants

pubmed: 32457730
doi: 10.3389/fmicb.2020.00876
pmc: PMC7225587
doi:

Types de publication

Journal Article

Langues

eng

Pagination

876

Informations de copyright

Copyright © 2020 Singh, Upadhyay, Singh and Panda.

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Auteurs

Anupam Singh (A)

Product Development Cell, National Institute of Immunology, New Delhi, India.

Vaibhav Upadhyay (V)

Product Development Cell, National Institute of Immunology, New Delhi, India.

Akansha Singh (A)

Product Development Cell, National Institute of Immunology, New Delhi, India.

Amulya K Panda (AK)

Product Development Cell, National Institute of Immunology, New Delhi, India.

Classifications MeSH