Physical and biological properties of blend-electrospun polycaprolactone/chitosan-based wound dressings loaded with N-decyl-N, N-dimethyl-1-decanaminium chloride: An in vitro and in vivo study.
Animals
Anti-Bacterial Agents
/ pharmacology
Bandages
Cell Adhesion
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
Cells, Cultured
Chitosan
Epithelium
/ drug effects
Gram-Positive Bacteria
/ drug effects
Humans
Hydrophobic and Hydrophilic Interactions
Mice
Mice, Inbred BALB C
Nanofibers
Neovascularization, Physiologic
/ drug effects
Polyesters
Porosity
Wound Healing
/ drug effects
Wounds and Injuries
/ drug therapy
N-decyl-N
N-dimethyl-1-decanaminium chloride
Nanofiber
chitosan
hydrophobic
polycaprolactone
wound dressing
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
19
12
2019
revised:
16
03
2020
accepted:
29
04
2020
pubmed:
28
5
2020
medline:
9
11
2021
entrez:
28
5
2020
Statut:
ppublish
Résumé
Dual-pump electrospinning of antibacterial N-decyl-N, N-dimethyl-1-decanaminium-chloride (DDAC)-loaded polycaprolactone (PCL) nanofibers, and chitosan (CS)/polyethylene-oxide (PEO)-based wound dressings with hydrophilic and hydrophobic properties to eliminate and absorb pathogenic bacteria from wound surface besides antibacterial action and to support wound healing and accelerate its process. Physicochemical properties of the prepared nanofibrous mat as well as antibacterial, cytotoxicity, and cell compatibility were studied. The full-thickness excisional wound healing properties up to 3 weeks using hematoxylin and eosin and Masson-trichrome staining were investigated. Addition of DDAC to CS/PEO-PCL mats decreased the diameter of the nanofibers, which is a crucial property for wound healing as large surface area per volume ratio of nanofibers, in addition to proper cell adhesion, increases loading of DDAC in mats and leads to increased cell viability and eliminating Gram-positive bacteria at in vitro studies. In vivo studies showed DDAC-loaded CS/PEO-PCL mats increased epithelialization and angiogenesis and decreased the inflammation according to histological results. We demonstrated that hydrophobic PCL/DDAC mats, besides antibacterial properties of DDAC, absorbed and eliminated the hydrophobic pathological microorganisms, whereas the hydrophilic nanofibers consisted of CS/PEO, increased the cell adhesion and proliferation due to positive charge of CS. Finally, we were able to increase the wound healing quality by using multifunctional wound dressing. CS/PEO-PCL containing 8 wt % of DDAC nanofibrous mats is promising as a wound dressing for wound management due to the favorable interactions between the pathogenic bacteria and PCL/CS-based wound dressing.
Substances chimiques
Anti-Bacterial Agents
0
Polyesters
0
polycaprolactone
24980-41-4
Chitosan
9012-76-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3084-3098Informations de copyright
© 2020 Wiley Periodicals, Inc.
Références
Abdali, Z., Logsetty, S., & Liu, S. (2019). Bacteria-responsive single and core-shell nanofibrous membranes based on polycaprolactone/poly(ethylene succinate) for on-demand release of biocides. ACS Omega, 4(2), 4063-4070.
Adam, A. B., Özdamar, M. Y., Esen, H. H., & Günel, E. (2017). Local effects of epidermal growth factor on the wound healing in esophageal anastomosis: An experimental study. International Journal of Pediatric Otorhinolaryngology, 99, 8-12. Retrieved from. https://linkinghub.elsevier.com/retrieve/pii/S0165587617302173
Asri, L. A. T. W., Crismaru, M., Roest, S., Chen, Y., Ivashenko, O., Rudolf, P., … Busscher, H. J. (2014). A shape-adaptive, antibacterial-coating of immobilized quaternary-ammonium compounds tethered on hyperbranched polyurea and its mechanism of action. Advanced Functional Materials, 24, 346-355.
Bayat, S., Amiri, N., Pishavar, E., Kalalinia, F., Movaffagh, J., & Hahsemi, M. (2019). Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models. Life Sciences, 229, 57-66.
Bettega, D., Calzolari, P., Doglia, S. M., Dulio, B., Tallone, L., & Villa, A. M. (1998). Cell thickness measurements by confocal fluorescence microscopy on C3H10T1/2 and V79 cells. International Journal of Radiation Biology, 74(3), 397-403.
Butler, M. F., Ng, Y.-F., & Pudney, P. D. A. (2003). Mechanism and kinetics of the crosslinking reaction between biopolymers containing primary amine groups and genipin. Journal of Polymer Science, Part A: Polymer Chemistry, 41(24), 3941-3953. https://doi.org/10.1002/pola.10960
Chang, H.-I., & Wang, Y. (2012). Cell responses to surface and architecture of tissue engineering scaffolds. In Regenerative medicine and tissue engineering-Cells and biomaterials, (569-588). London, England: INTECHOPEN LIMITED. https://www.intechopen.com/books/regenerative-medicine-and-tissue-engineering-cells-and-biomaterials/cell-responses-to-surface-and-architecture-of-tissue-engineering-scaffolds.
Chantre, C. O., Campbell, P. H., Golecki, H. M., Buganza, A. T., Capulli, A. K., Deravi, L. F., … Parker, K. K. (2018). Production-scale fibronectin nanofibers promote wound closure and tissue repair in a dermal mouse model. Biomaterials, 166, 96-108. https://doi.org/10.1016/j.biomaterials.2018.03.006
Dallaire, C., Kolber, I., & Gingras, M. (2002). Nickel-catalyzed coupling of aryl o-carbamates with grignard reagents: 2,7-dimethylnaphthalene. Organic Syntheses, 78, 42. Retrieved from. http://orgsyn.org/demo.aspx?prep=V78P0042
Doyle, R. J. (2000). Contribution of the hydrophobic effect to microbial infection. Microbes and Infection, 2(4), 391-400. Retrieved from. http://www.ncbi.nlm.nih.gov/pubmed/10817641
Esmati, N., Khodaei, T., Salahinejad, E., & Sharifi, E. (2018). Fluoride doping into SiO2-MgO-CaO bioactive glass nanoparticles: Bioactivity, biodegradation and biocompatibility assessments. Ceramics International, 44, 17506-17513.
Farhood, A. H., & Chelab, R. L. (2017). Isolation and identification of bacteria from burn injuries. Journal of College of Education for Pure Sciences, 7(1), 126-139.
Faris, A., Wadström, T., & Freer, J. H. (1981). Hydrophobic adsorptive and hemagglutinating properties Ofescherichia coli possessing colonization factor antigens (CFA/I or CFA/II), type 1 pili, or other pili. Current Microbiology, 5(2), 67-72. Retrieved from. http://link.springer.com/10.1007/BF01567421
Feldman, W. H., Karlson, A. G., & Herrick, J. F. (1957). Mycobacterium ulcerans. The American Journal of Pathology, 33(6), 1163-1179.
Gomes, S. R., Rodrigues, G., Martins, G. G., Roberto, M. A., Mafra, M., Henriques, C. M. R., & Silva, J. C. (2015). In vitro and in vivo evaluation of electrospun nano fi bers of PCL, chitosan and gelatin: A comparative study. Materials Science and Engineering C, 46, 348-358. https://doi.org/10.1016/j.msec.2014.10.051
Goodarzi, H., Jadidi, K., Pourmotabed, S., Sharifi, E., & Aghamollaei, H. (2018). Preparation and in vitro characterization of cross-linked collagen-gelatin hydrogel using EDC/NHS for corneal tissue engineering applications. International Journal of Biological Macromolecules, 126, 620-632.
Gottrup, F., Agren, M. S., & Karlsmark, T. (2018). Models for use in wound healing research: A survey focusing on in vitro and in vivo adult soft tissue. Wound Repair and Regeneration, 8(2), 83-96. Retrieved from. http://www.ncbi.nlm.nih.gov/pubmed/10810034
Guarino, V., Causa, F., Taddei, P., di Foggia, M., Ciapetti, G., Martini, D., … Ambrosio, L. (2008). Polylactic acid fibre-reinforced polycaprolactone scaffolds for bone tissue engineering. Biomaterials, 29(27), 3662-3670. Retrieved from. http://www.ncbi.nlm.nih.gov/pubmed/18547638
Guha, A., Nayar, S., & Thatoi, H. N. (2010). Microwave irradiation enhances kinetics of the biomimetic process of hydroxyapatite nanocomposites. Bioinspiration & Biomimetics, 5(2):024001.
Han, D., & Steckl, A. J. (2009). Superhydrophobic and oleophobic fibers by coaxial electrospinning. Langmuir, 25(16), 9454-9462.
Johnson, J. L., Najor, N. A., & Green, K. J. (2014). Desmosomes: Regulators of cellular signaling and adhesion in epidermal health and disease. Cold Spring Harbor Perspectives in Medicine, 4(11):015297.
Jung, J. A., Yoo, K. H., Han, S. K., Lee, Y. N., Jeong, S. H., Dhong, E. S., & Kim, W. K. (2016). Influence of negative-pressure wound therapy on tissue oxygenation in diabetic feet. Advances in Skin & Wound Care, 29(8), 364-370.
Kamel, N. A., Soliman, A. A. F., Rozik, N. N., & Abd-Elmessieha, S. L. (2018). Biophysical investigation of curcumin based nanocomposite for wound dressing application. Journal of Applied Pharmaceutical Science, 8(5), 35-44.
Kammerlander, G., Locher, E., Suess-Burghart, A., & Wipplinger, P. (2008). An investigation of Cutimed® Sorbact® as an antimicrobial alternative in wound management. Wounds UK, 4, 10-18. Retrieved from. https://www.wounds-uk.com/journals/issue/14/article-details/international-conferences-give-us-the-chance-to-bring-the-wound-care-message-home-1
Karimi, S., Salahinejad, E., Shari, E., Nourian, A., & Tayebi, L. (2018). Bioperformance of chitosan/fluoride-doped diopside nanocomposite coatings deposited on medical stainless steel. Carbohydrate Polymers, 202, 600-610.
Kim, S. S., & Lee, J. (2014). Antibacterial activity of polyacrylonitrile-chitosan electrospun nanofibers. Carbohydrate Polymers, 102(1), 231-237. https://doi.org/10.1016/j.carbpol.2013.11.028
Kourmouli, A., Valenti, M., van Rijn, E., Beaumont, H. J. E., Kalantzi, O.-I., Schmidt-Ott, A., & Biskos, G. (2018). Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles? Journal of Nanoparticle Research, 20(3), 62. https://doi.org/10.1007/s11051-018-4152-3
Lee, K. Y., Jeong, L., Kang, Y. O., Lee, S. J., & Park, W. H. (2009). Electrospinning of polysaccharides for regenerative medicine. Advanced Drug Delivery Reviews, 61(12), 1020-1032 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19643155.
Levina, E. M., Kharitonova, M. A., Rovensky, Y. A., & Vasiliev, J. M. (2001). Cytoskeletal control of fibroblast length: Experiments with linear strips of substrate. Journal of Cell Science, 114(23), 4335-4341.
Li, X., Wang, C., Yang, S., Liu, P., & Zhang, B. (2018). Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications. International Journal of Nanomedicine, 13, 5287-5299.
Liang, D., Hsiao, B. S., & Chu, B. (2007). Functional electrospun nanofibrous scaffolds for biomedical applications. Advanced Drug Delivery Reviews, 59(14), 1392-1412 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/17884240.
Licheng Ren, M. D., Bo Zhou, M. D., & Lei, C. M. (2012). Silicone ring implantation in an excisional murine wound model. Wounds, 24, 36-42.
Ljungh, Å., Yanagisawa, N., & Wadström, T. (2014). Using the principle of hydrophobic interaction to bind and remove wound bacteria. Journal of Wound Care, 15(4), 175-180 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16620048.
Markova, I. N., Zahariev, I. Z., Milanova, V. L., Ivanova, D. I., Piskin, M. B., Fachikov, L. B., & Hristoforou, E, et al. (2017). Nanomaterials based on intermetallic (Co-Sn, Ni-Sn, Co-Ni) nanoparticles studied by ftir spectroscopy. Reviews on Advanced Materials Science, 52(1-2), 70-81.
Masoodi, R., & Pillai, K. M. (2012). A general formula for capillary suction-pressure in porous media. Journal of Porous Media, 15(8), 775-783 Retrieved from http://www.dl.begellhouse.com/journals/49dcde6d4c0809db,7d0fc4c5314ecd78,6d3ceea5522b464a.html.
Mohan, P. R. K., Sreelakshmi, G., Muraleedharan, C. V., & Joseph, R. (2012). Water soluble complexes of curcumin with cyclodextrins: Characterization by FT-Raman spectroscopy. Vibrational Spectroscopy, 62, 77-84 Retrieved from https://www.sciencedirect.com/science/article/abs/pii/S092420311200118X.
Moulin, V., Auger, F. A., Garrel, D., & Germain, L. (2000). Role of wound healing myofibroblasts on re-epithelialization of human skin. Burns, 26(1), 3-12 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10630313.
Muñoz-Bonilla, A., & Fernández-García, M. (2012). Polymeric materials with antimicrobial activity. Progress in Polymer Science, 37(2), 281-339.
Muzzarelli, R. A. A. (2009). Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 76(2), 167-182 Retrieved from https://linkinghub.elsevier.com/retrieve/pii/S0144861708005183.
Nosrati, H., Pourmotabed, S., & Sharifi, E. (2018). A review on some natural biopolymers and their applications in angiogenesis and tissue engineering. Journal of Applied Biotechnology Reports, 5(3), 81-91 Retrieved from http://www.biotechrep.ir/article_80643.html.
Osman, Z., & Arof, A. K. (2003). FTIR studies of chitosan acetate based polymer electrolytes. Electrochimica Acta, 48(8), 993-999.
Perez, R., & Davis, S. C. (2008). Relevance of animal models for wound healing. Wounds, 20(1), 3-8 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/25942755.
Qin, Z., Balimunkwe, R. M., & Quan, T. (2017). Age-related reduction of dermal fibroblast size upregulates multiple matrix metalloproteinases as observed in aged human skin in vivo. The British Journal of Dermatology, 177(5), 1337-1348.
Queiroz, M. F., Melo, K. R. T., Sabry, D. A., Sassaki, G. L., & Rocha, H. A. O. (2015). Does the use of chitosan contribute to oxalate kidney stone formation? Marine Drugs, 13(1), 141-158.
Rahmani Del Bakhshayesh, A., Annabi, N., Khalilov, R., Akbarzadeh, A., Samiei, M., Alizadeh, E., … Montaseri, A. (2018). Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering. Artif Cells, Nanomedicine, Biotechnology, 46(4), 691-705 Retrieved from https://www.tandfonline.com/doi/full/10.1080/21691401.2017.1349778.
Reinke, J. M., & Sorg, H. (2012). Wound repair and regeneration. European Surgical Research, 49(1), 35-43.
Shalumon, K. T., Anulekha, K. H., Chennazhi, K. P., Tamura, H., Nair, S. V., & Jayakumar, R. (2011). Fabrication of chitosan/poly(caprolactone) nanofibrous scaffold for bone and skin tissue engineering. International Journal of Biological Macromolecules, 48(4), 571-576. https://doi.org/10.1016/j.ijbiomac.2011.01.020
Shan, Y. H., Peng, L. H., Liu, X., Chen, X., Xiong, J., & Gao, J. Q. (2015). Silk fibroin/gelatin electrospun nanofibrous dressing functionalized with astragaloside IV induces healing and anti-scar effects on burn wound. International Journal of Pharmaceutics, 479(2), 291-301. https://doi.org/10.1016/j.ijpharm.2014.12.067
Sharifi, E., Ebrahimi-Barough, S., Panahi, M., Azami, M., Ai, A., Barabadi, Z., Kajbafzadeh, A., & Ai, J., et al. (2016). In vitro evaluation of human endometrial stem cell-derived osteoblast-like cells' behavior on gelatin/collagen/bioglass nanofibers' scaffolds. Journal of Biomedical Materials Research Part A, 104(9), 2210-2219.
Shi, Q., Luo, X., Huang, Z., Midgley, A. C., Wang, B., Liu, R., … Wang, K. (2019). Cobalt-mediated multi-functional dressings promote bacteria-infected wound healing. Acta Biomaterialia, 86, 465-479. https://doi.org/10.1016/j.actbio.2018.12.048
Shi, R., Geng, H., Gong, M., Ye, J., Wu, C., Hu, X., & Zhang, L. (2018). Long-acting and broad-spectrum antimicrobial electrospun poly (ε-caprolactone)/gelatin micro/nanofibers for wound dressing. Journal of Colloid and Interface Science, 509, 275-284. https://doi.org/10.1016/j.jcis.2017.08.092
Siedlecka, A., & Piekarska, K. (2017). The evaluation of the classical Kirby-Bauer antibiogram method for the determination of antibiotic resistant microorganisms in tap water. E3S Web of Conferences, 17, 00081.
Sisay, M., Worku, T., & Edessa, D. (2019). Microbial epidemiology and antimicrobial resistance patterns of wound infection in Ethiopia: A meta-analysis of laboratory-based cross-sectional studies. BMC Pharmacology and Toxicology, 20(1).35-54.
Sullivan, T. P., Eaglstein, W. H., Davis, S. C., & Mertz, P. (2001). The pig as a model for human wound healing. Wound Repair and Regeneration, 9(2), 66-76 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11350644.
Sun, Y., & Sun, G. (2003). Novel refreshable N-halamine polymeric biocides: Grafting hydantoin-containing monomers onto high performance fibers by a continuous process. Journal of Applied Polymer Science, 88(4), 1032-1039.
Tao, G., Wang, Y., Cai, R., Chang, H., Song, K., Zuo, H., … He, H. (2019). Design and performance of sericin/poly(vinyl alcohol) hydrogel as a drug delivery carrier for potential wound dressing application. Materials Science and Engineering: C, 101, 341-351. https://doi.org/10.1016/j.msec.2019.03.111
Vadillo-Rodríguez, V., Busscher, H. J., Norde, W., de Vries, J., & van der Mei, H. C. (2004). Dynamic cell surface hydrophobicity of lactobacillus strains with and without surface layer proteins. Journal of Bacteriology, 186(19), 6647-6650 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/15375147.
Wang, X., Ge, J., Tredget, E. E., & Wu, Y. (2013). The mouse excisional wound splinting model, including applications for stem cell transplantation. Nature Protocols, 8(2), 302-309.
Wolpert, M., & Hellwig, P. (2006). Infrared spectra and molar absorption coefficients of the 20 alpha amino acids in aqueous solutions in the spectral range from 1800 to 500 cm−1. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 64(4), 987-1001 Retrieved from https://www.sciencedirect.com/science/article/pii/S1386142505005044.
Xue, W., Lv, C., Jing, Y., Chen, F., & Fu, Q. (2017). Fabrication of electrospun PVDF nanofibers with higher content of polar β phase and smaller diameter by adding a small amount of dioctadecyl dimethyl ammonium chloride. Chinese Journal of Polymer Science, 35(8), 992-1000.
Yahata, Y., Shirakata, Y., Tokumaru, S., Yang, L., Dai, X., Tohyama, M., … Hashimoto, K. (2006). A novel function of angiotensin II in skin wound healing. The Journal of Biological Chemistry, 281(19), 13209-13216 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16543233.
Zhang, G., Qu, J., Liu, H., Cooper, A. T., & Wu, R. (2007). CuFe2O4/activated carbon composite: A novel magnetic adsorbent for the removal of acid orange II and catalytic regeneration. Chemosphere, 68(6), 1058-1066 Retrieved from https://www.sciencedirect.com/science/article/pii/S0045653507002007.
Zheng, Z., Liu, Y., Huang, W., Mo, Y., Lan, Y., Guo, R., & Cheng, B. (2018). Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing. Artificial Cells, Nanomedicine, and Biotechnology, 46, 493-501. https://doi.org/10.1080/21691401.2018.1460372
Zhu, X., Cui, W., Li, X., & Jin, Y. (2008). Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules, 9(7), 1795-1801.