[Development of a set of reagents for detection of class G immunoglobulins to human herpes virus type 7 by the method of immune blotting in the format «Western blot».]
enzyme immunoassay (ELISA)
g-class immunoglobulins (IgG)
human herpes virus type 6
human herpes virus type 7 (HCV-7)
immune blotting (IB)
Journal
Klinicheskaia laboratornaia diagnostika
ISSN: 0869-2084
Titre abrégé: Klin Lab Diagn
Pays: Russia (Federation)
ID NLM: 9432021
Informations de publication
Date de publication:
2020
2020
Historique:
received:
18
03
2020
accepted:
29
03
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
17
9
2020
Statut:
ppublish
Résumé
A new original domestic set of reagents has been developed for the determination of class G immunoglobulins to individual human herpes virus antigens of type 7 by the method of immune blotting in the "Western-blot" format. Preliminary clinical trials were conducted using 134 serums of healthy children aged 1-16 years who underwent diagnostic testing. Stud y of diagnostic efficiency ofthe new kit showed high sensitivity, comparable to the sensitivity of the reaction indirect immunofluorescence and high specificity, which is manifested in the absence of false positive results when testing samples containing immunoglobulin G to herpes virus 6 type.
Identifiants
pubmed: 32459895
doi: 10.18821/0869-2084-2020-65-6-362-367
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin G
0
Indicators and Reagents
0
Types de publication
Journal Article
Langues
rus
Sous-ensembles de citation
IM
Pagination
362-367Déclaration de conflit d'intérêts
The authors declare no conflict of interest.