Individual Control and Quantification of 3D Spheroids in a High-Density Microfluidic Droplet Array.
droplet microfluidics
liver toxicity
organoids
spheroids
tissue engineering
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
26 05 2020
26 05 2020
Historique:
received:
07
05
2019
revised:
20
11
2019
accepted:
29
04
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
22
5
2021
Statut:
ppublish
Résumé
As three-dimensional cell culture formats gain in popularity, there emerges a need for tools that produce vast amounts of data on individual cells within the spheroids or organoids. Here, we present a microfluidic platform that provides access to such data by parallelizing the manipulation of individual spheroids within anchored droplets. Different conditions can be applied in a single device by triggering the merging of new droplets with the spheroid-containing drops. This allows cell-cell interactions to be initiated for building microtissues, studying stem cells' self-organization, or observing antagonistic interactions. It also allows the spheroids' physical or chemical environment to be modulated, as we show by applying a drug over a large range of concentrations in a single parallelized experiment. This convergence of microfluidics and image acquisition leads to a data-driven approach that allows the heterogeneity of 3D culture behavior to be addressed across the scales, bridging single-cell measurements with population measurements.
Identifiants
pubmed: 32460010
pii: S2211-1247(20)30623-9
doi: 10.1016/j.celrep.2020.107670
pmc: PMC7262598
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107670Subventions
Organisme : European Research Council
ID : 278248
Pays : International
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests R.F.-X.T., S.S., and C.N.B. are co-inventors of two patents related to this work, owned by CNRS and Ecole Polytechnique.
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