Interleukin-13 induces paralysis of Haemonchus contortus larvae in vitro.
Haemonchus contortus
Interleukin-13
helminth
immunity
monocytes
motility
ovine
Journal
Parasite immunology
ISSN: 1365-3024
Titre abrégé: Parasite Immunol
Pays: England
ID NLM: 7910948
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
17
02
2020
revised:
15
05
2020
accepted:
19
05
2020
pubmed:
28
5
2020
medline:
12
2
2021
entrez:
28
5
2020
Statut:
ppublish
Résumé
Interleukin-13 (IL-13) is a Th2-associated cytokine that typically induces gut contractility and mucus secretion to eliminate helminth parasites from the digestive tract. Little evidence exists of IL-13's direct effect on Haemonchus contortus larvae (L3) and thus was the objective of this study. To test effects of IL-13 on H contortus, L3 were treated with ovine recombinant (r) IL-13 (1 μg/mL); motility and morbidity were assessed. Monocytes isolated from H contortus-resistant St. Croix (STC) and susceptible Suffolk (SUF) sheep were treated with anti-IL-13 blocking antibody to elucidate differences in host immune response. rIL-13 treatment reduced L3 speed (27 μm/s) and distance (7.5 μm) compared to untreated L3 (speed: 94 μm/s; distance: 27 μm) (P < .001). Comparison of larval speed to known paralytic levamisole (LEV) revealed no difference between treatments (rIL13: 23 μm/s; LEV 27 μm/s). Additionally, rIL-13 had no effect on larval morbidity. Blocking IL-13 reduced monocyte-driven larval morbidity (0.13 μmol/L ATP) and increased larval motility (88 μm/s; 27 μm) compared to larvae treated with STC-monocytes alone (0.07 μM ATP; 34 μm/s; 8 μm) (P < .05). These data indicate IL-13 has a dual capability paralysing L3 and contributing to monocyte-driven larval morbidity, and also indicate breed differences.
Substances chimiques
Interleukin-13
0
Types de publication
Journal Article
Randomized Controlled Trial, Veterinary
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12758Informations de copyright
© 2020 John Wiley & Sons Ltd.
Références
Stanley AC, Lacy P. Pathways for cytokine secretion. Physiology. 2010;25(4):218-229.
Anthony RM, Rutitzky LI, Urban JF, Stadecker MJ, Gause WC. Protective immune mechanisms in helminth infection. Nat Rev Immunol. 2007;7(12):975-987.
Wynn TA. IL-13 effector functions. Annu Rev Immunol. 2003;21(1):425-456.
Barner M, Mohrs M, Brombacher F, Kopf M. Differences between IL-4Rα-deficient and IL-4-deficient mice reveal a role for IL-13 in the regulation of Th2 responses. Curr Biol. 1998;8(11):669-672.
Urban JF, Noben-Trauth N, Schopf L, Madden KB, Finkelman FD. Cutting edge: IL-4 receptor expression by non-bone marrow-derived cells is required to expel gastrointestinal nematode parasites. J Immunol. 2001;167(11):6078-6081.
Martin JR. Modes of action of anthelmintic drugs. Vet J. 1997;154:11-34.
Georgi JR, Whitelock JH. Erythrocyte loss and resitution in ovine haemonchosis. Estimation of erythrocyte loss in lambs following natural exposure. Cornell Vet. 1967;57(1):44-53.
Chen F, Wu W, Millman A, et al. Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion. Nat Immunol. 2014;15(10):938-946.
Bonne-Année S, Kerepesi LA, Hess JA, et al. Human and mouse macrophages collaborate with neutrophils to kill larval strongyloides stercoralis. Infect Immun. 2013;81(9):3346-3355.
McKenzie GJ, Bancroft AJ, Grencis RK, McKenzie ANJ. A distinct role for interleukin-13 in Th2-cell-mediated immune responses. Curr Biol. 1998;8(6):339-342.
MacKinnon KM, Zajac AM, Kooyman FNJ, Notter DR. Differences in immune parameters are associated with resistance to Haemonchus contortus in Caribbean hair sheep. Parasite Immunol. 2010;32(7):484-493.
Bowdridge SA, Zajac AM, St NDR. Croix sheep produce a rapid and greater cellular immune response contributing to reduced establishment of Haemonchus contortus. Vet Parasitol. 2015;208(3-4):204-210.