Capillary electrophoresis and ultra-high-performance liquid chromatography methods in clinical monitoring of creatinine in human urine: A comparative study.
capillary isotachophoresis
capillary zone electrophoresis
clinical analysis
creatinine
mass spectrometry
ultra-high-performance liquid chromatography
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
02
04
2020
revised:
21
05
2020
accepted:
25
05
2020
pubmed:
28
5
2020
medline:
27
4
2021
entrez:
28
5
2020
Statut:
ppublish
Résumé
Creatinine is an important diagnostic marker and is also used as a standardization tool for the quantitative evaluation of exogenous/endogenous substances in urine. This study aimed at evaluating and comparing three analytical approaches, based on hyphenations of different separation [two-dimensional capillary isotachophoresis (CITP-CITP), capillary zone electrophoresis (CZE), ultra-high-performance liquid chromatography (UHPLC)] and detection [conductivity (CD), ultraviolet (UV), tandem mass spectrometry (MS/MS)] techniques, for their ability to provide reliable clinical data along with their suitability for the routine clinical use (cost, simplicity, sample throughput). The developed UHPLC-MS/MS, CITP-CITP-CD, and CZE-UV methods were characterized by favorable performance parameters, such as linearity (r ˃ 0.99), precision (relative standard deviation, 0.22-2.97% for the creatinine position in analytical profiles), and recovery (87.1-115.1%). Clinical data, obtained from the analysis of 24 human urine samples by a reference enzymatic method, were comparable with those obtained by the tested methods (Passing-Bablok regression and Bland-Altman analysis), approving their usefulness for the routine clinical use. In this context, the UHPLC-MS/MS method provides benefits of enhanced orthogonality/accuracy and high sample throughput (threefold shorter total analysis times than the CE methods), whereas advantages of the CE methods for routine labs are simplicity and low cost of both the instrumentation and measurements.
Substances chimiques
Creatinine
AYI8EX34EU
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4907Subventions
Organisme : Agentúra na Podporu Výskumu a Vývoja
ID : APVV-15-0585
Organisme : Kultúrna a Edukačná Grantová Agentúra MŠVVaŠ SR
ID : KEGA 027UK-4/2020
Organisme : Vedečká Grantová Agentúra MŠVVaŠ SR a SAV
ID : VEGA 1/0463/18
Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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