Imaging of lysophosphatidylcholine in an induced pluripotent stem cell-derived endothelial cell network.
Endothelial
Imaging
Lysophosphatidylcholine
MALDI
Pluripotent
Stem
Journal
Regenerative therapy
ISSN: 2352-3204
Titre abrégé: Regen Ther
Pays: Netherlands
ID NLM: 101709085
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
11
01
2020
revised:
01
03
2020
accepted:
11
03
2020
entrez:
29
5
2020
pubmed:
29
5
2020
medline:
29
5
2020
Statut:
epublish
Résumé
Vascular endothelial cell disorders are closely related to cardiovascular disease (CVD) and pulmonary diseases. Abnormal lipid metabolism in the endothelium leads to changes in cell signalling, and the expression of genes related to immunity and inflammation. It is therefore important to investigate the pathophysiology of vascular endothelial disorders in terms of lipid metabolism, using a disease model of endothelium. Human induced pluripotent stem cell-derived endothelial cells (iECs) were cultured on a matrigel to form an iEC network. Lipids in the iEC network were investigated by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) imaging mass spectrometry (IMS) analysis. Ion fragments obtained by mass spectrometry were analysed using an infusion method, involving precursor ion scanning with fragment ion. The MALDI TOF IMS analysis revealed co-localized intensity of peaks at The MALDI-imaging analysis showed that LPC (22:5) was abundant in an iEC network. As an in vitro test model for disease and potential therapy, present analysis methods using MALDI-imaging combined with, for example, mesenchymal stem cells (MSC) to a disease derived iEC network may be useful in revealing the changes in the amount and distribution of lipids under various stimuli.
Identifiants
pubmed: 32462058
doi: 10.1016/j.reth.2020.03.007
pii: S2352-3204(20)30028-6
pmc: PMC7240204
doi:
Types de publication
Journal Article
Langues
eng
Pagination
299-305Informations de copyright
© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
Déclaration de conflit d'intérêts
None of the authors have any conflict of interests.
Références
J Lipid Res. 2011 May;52(5):1011-22
pubmed: 21335323
Mol Cell Proteomics. 2012 Feb;11(2):M111.010694
pubmed: 22084000
Am J Respir Crit Care Med. 2017 Apr 1;195(7):930-941
pubmed: 27779452
J Proteome Res. 2019 Apr 5;18(4):1669-1678
pubmed: 30784274
Biochem Biophys Res Commun. 2018 Oct 7;504(3):iii
pubmed: 30293583
Nature. 2017 Aug 30;548(7669):592-596
pubmed: 28858313
Anal Bioanal Chem. 2019 Jun;411(15):3221-3227
pubmed: 31037373
Physiology (Bethesda). 2017 Mar;32(2):126-140
pubmed: 28202623
Respir Res. 2018 Jan 10;19(1):7
pubmed: 29321022
Mass Spectrom Rev. 2018 Mar;37(2):107-138
pubmed: 27276657
Am Soc Clin Oncol Educ Book. 2019 Jan;39:165-174
pubmed: 31099649
Metabolism. 1996 May;45(5):559-64
pubmed: 8622597
Sci Rep. 2018 Aug 17;8(1):12365
pubmed: 30120318
Physiol Rev. 2019 Jan 1;99(1):79-114
pubmed: 30328784
Mass Spectrom Rev. 2010 Nov-Dec;29(6):877-929
pubmed: 20931646
J Mol Cell Cardiol. 2003 Nov;35(11):1375-84
pubmed: 14596794
Anal Chem. 2019 Mar 5;91(5):3575-3581
pubmed: 30702282
J Proteome Res. 2014 Sep 5;13(9):3919-29
pubmed: 25040188
Sci Rep. 2018 Aug 23;8(1):12677
pubmed: 30140012
Rapid Commun Mass Spectrom. 2004;18(24):3123-30
pubmed: 15565732
Sci Rep. 2017 Aug 24;7(1):9377
pubmed: 28839272
Oncotarget. 2017 Nov 10;8(63):106177-106189
pubmed: 29290940
Redox Biol. 2019 Jun;24:101221
pubmed: 31153039
Adv Exp Med Biol. 2019;1159:109-138
pubmed: 31502202
Annu Rev Physiol. 2019 Feb 10;81:505-534
pubmed: 30742782
Biochem Biophys Res Commun. 2018 Oct 7;504(3):590-595
pubmed: 29665359
Fluids Barriers CNS. 2018 Apr 4;15(1):9
pubmed: 29615068
Regen Ther. 2019 Jun 13;11:75-80
pubmed: 31245451
Sci Rep. 2019 May 15;9(1):7414
pubmed: 31092842
PLoS Genet. 2014 Dec 11;10(12):e1004801
pubmed: 25502724
J Am Soc Mass Spectrom. 2018 Feb;29(2):316-322
pubmed: 28755258
Oncol Lett. 2017 Mar;13(3):1204-1210
pubmed: 28454235
Proteomics Clin Appl. 2018 May;12(3):e1700086
pubmed: 29283216
Sci Rep. 2019 Sep 13;9(1):13213
pubmed: 31519997
J Proteome Res. 2015 Jun 5;14(6):2511-9
pubmed: 25880480
Anal Bioanal Chem. 2017 Feb;409(4):1007-1016
pubmed: 27815610
Regen Ther. 2019 May 10;11:25-30
pubmed: 31193176
J Appl Physiol (1985). 2019 Aug 1;127(2):444-456
pubmed: 31295064
Am J Respir Cell Mol Biol. 2016 Feb;54(2):231-40
pubmed: 26161876
Data Brief. 2015 Jun 24;4:328-31
pubmed: 26217810
Biochem Biophys Res Commun. 2018 Oct 7;504(3):576-581
pubmed: 29534960
PLoS One. 2014 Jan 17;9(1):e85724
pubmed: 24465667
Aging Cell. 2019 Dec;18(6):e13048
pubmed: 31560163
J Biol Chem. 2018 Jul 13;293(28):11033-11045
pubmed: 29769317
Adv Exp Med Biol. 2020;1212:49-56
pubmed: 31228130
Int J Mol Sci. 2019 Mar 06;20(5):
pubmed: 30845751
N Engl J Med. 2017 Aug 24;377(8):792-793
pubmed: 28834478
Circ Res. 2016 Feb 19;118(4):620-36
pubmed: 26892962
Neuroscience. 2010 Jun 16;168(1):219-25
pubmed: 20362643
FASEB J. 2016 Jun;30(6):2435-50
pubmed: 27006447