The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
Betacoronavirus
/ genetics
COVID-19
COVID-19 Testing
Clinical Laboratory Techniques
/ methods
Coronavirus Envelope Proteins
Coronavirus Infections
/ diagnosis
Coronavirus Nucleocapsid Proteins
Coronavirus RNA-Dependent RNA Polymerase
France
Humans
Nasopharynx
/ virology
Nucleocapsid Proteins
/ genetics
Pandemics
Phosphoproteins
Pneumonia, Viral
/ diagnosis
Prospective Studies
RNA-Dependent RNA Polymerase
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
/ methods
SARS-CoV-2
Viral Envelope Proteins
/ genetics
Viral Nonstructural Proteins
/ genetics
Allplex 2019-nCoV (Seegene)
COVID-19
Nasopharyngeal swab
RT-PCR
SARS-CoV-2
Sensitivity
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
26
04
2020
accepted:
13
05
2020
pubmed:
29
5
2020
medline:
24
9
2020
entrez:
29
5
2020
Statut:
ppublish
Résumé
Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets.
Identifiants
pubmed: 32462501
doi: 10.1007/s10096-020-03930-8
pii: 10.1007/s10096-020-03930-8
pmc: PMC8824685
doi:
Substances chimiques
Coronavirus Envelope Proteins
0
Coronavirus Nucleocapsid Proteins
0
Nucleocapsid Proteins
0
Phosphoproteins
0
Viral Envelope Proteins
0
Viral Nonstructural Proteins
0
envelope protein, SARS-CoV-2
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
Coronavirus RNA-Dependent RNA Polymerase
EC 2.7.7.48
NSP12 protein, SARS-CoV-2
EC 2.7.7.48
RNA-Dependent RNA Polymerase
EC 2.7.7.48
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1997-2000Références
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