Impact of the sexual abstinence period on the production of seminal reactive oxygen species in patients undergoing intrauterine insemination: A randomized trial.
intrauterine insemination, semen analysis
oxidative stress
oxisperm
sexuel abstinence
Journal
The journal of obstetrics and gynaecology research
ISSN: 1447-0756
Titre abrégé: J Obstet Gynaecol Res
Pays: Australia
ID NLM: 9612761
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
20
04
2020
accepted:
30
04
2020
pubmed:
29
5
2020
medline:
22
6
2021
entrez:
29
5
2020
Statut:
ppublish
Résumé
To evaluate whether the duration of sexual abstinence has impact on oxidative stress in semen samples. Oxidative reaction was tested for different levels of reactive oxygen species (ROS) by nitro blue tetrazolium assay in 90 patients with the diagnosis of unexplained or male factor infertility that were grouped into 3 groups as 0-2 (group 1), 3-4 (group 2) and >4 days (group 3) of duration of sexual abstinence. Subsequently, the remaining semen was prepared by gradient method for ovarian stimulation and intrauterine insemination (IUI) cycles to compare pregnancy rates in terms of different levels of ROS and different abstinence periods. Increased staining pigment intensity was related to higher level of ROS in >4 days' group as compared to groups 0-2 and 3-4 days (70% vs 43.3% and 50%, P = 0.013 and P = 0.014, respectively). Pregnancy rates significantly decrease with prolonged abstinence period (26.7%, 16.7% and 6.7% in groups 1, 2 and 3, respectively, P = 0.039). Progressive motile sperm count after gradient method of sperm preparation for IUI was highest in 3-4 days of abstinence period than shorter and longer abstinence groups. Longer duration of sexual abstinence causes higher oxidative stress and decreases pregnancy rates in IUI cycles.
Substances chimiques
Reactive Oxygen Species
0
Types de publication
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Pagination
1133-1139Subventions
Organisme : Gazi University
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Japan Society of Obstetrics and Gynecology.
Références
Cooper TG, Noonan E, von Eckardstein Set al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010; 16: 231-245.
Sauer MV, Zeffer KB, Buster JE, Sokol RZ. Effect of abstinence on sperm motility in normal men. Am J Obstet Gynecol 1988; 158 (3 Pt 1): 604-607.
Pellestor F, Girardet A, Andreo B. Effect of long abstinence periods on human sperm quality. Int J Fertil Menopausal Stud 1994; 39: 278-282.
Marshburn PB, Alanis M, Matthews MLet al. A short period of ejaculatory abstinence before intrauterine insemination is associated with higher pregnancy rates. Fertil Steril 2010; 93: 286-288.
Kably-Ambe A, Carballo-Mondragon E, Duran-Monterrosas L, Soriano-Ortega KP, Roque-Sanchez AM. Effect of sexual abstinence on pregnancy rates after an intrauterine insemination. Ginecol Obstet Mex 2015; 83: 104-109.
Jurema MW, Vieira AD, Bankowski Bet al. Effect of ejaculatory abstinence period on the pregnancy rate after intrauterine insemination. Fertil Steril 2005; 84: 678-681.
Marshburn PB, Giddings A, Causby Set al. Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation. Fertil Steril 2014; 102: 705-710.
Saleh RA, Agarwal A, Nada EAet al. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril 2003; 79 (Suppl 3): 1597-1605.
World Health Organization. WHO laboratory manual for the examination and processing of human semen. Genève, Switzerland: WHO, 2010.
Baehner RL, Boxer LA, Davis J. The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes. Blood 1976; 48: 309-313.
Kemal Duru N, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 2000; 74: 1200-1207.
Schulte RT, Ohl DA, Sigman M, Smith GD. Sperm DNA damage in male infertility: Etiologies, assays, and outcomes. J Assist Reprod Genet 2010; 27: 3-12.
Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992; 57: 409-416.
Lanzafame FM, La Vignera S, Vicari E, Calogero AE. Oxidative stress and medical antioxidant treatment in male infertility. Reprod Biomed Online 2009; 19: 638-659.
Desai NR, Mahfouz R, Sharma R, Gupta S, Agarwal A. Reactive oxygen species levels are independent of sperm concentration, motility, and abstinence in a normal, healthy, proven fertile man: A longitudinal study. Fertil Steril 2010; 94: 1541-1543.
Thomson LK, Zieschang JA, Clark AM. Oxidative deoxyribonucleic acid damage in sperm has a negative impact on clinical pregnancy rate in intrauterine insemination but not intracytoplasmic sperm injection cycles. Fertil Steril 2011; 96: 843-847.
Venkatesh S, Singh G, Gupta NP, Kumar R, Deecaraman M, Dada R. Correlation of sperm morphology and oxidative stress in infertile men. Iranian J Reproduc Med 2009; 7(1): 29-34.
Agarwal A, Tvrda E, Sharma R. Relationship amongst teratozoospermia, seminal oxidative stress and male infertility. Reprod Biol Endocrinol 2014; 12: 45.
De Jonge C, LaFromboise M, Bosmans E, Ombelet W, Cox A, Nijs M. Influence of the abstinence period on human sperm quality. Fertil Steril 2004; 82: 57-65.
Elzanaty S, Malm J, Giwercman A. Duration of sexual abstinence: Epididymal and accessory sex gland secretions and their relationship to sperm motility. Hum Reprod 2005; 20: 221-225.
Levitas E, Lunenfeld E, Weiss Net al. Relationship between the duration of sexual abstinence and semen quality: Analysis of 9,489 semen samples. Fertil Steril 2005; 83: 1680-1686.
Sanchez-Martin P, Sanchez-Martin F, Gonzalez-Martinez M, Gosalvez J. Increased pregnancy after reduced male abstinence. Syst Biol Reprod Med 2013; 59: 256-260.