Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters.
RNA clusters
RNA granules
RNA phase separation
RNA self-assembly
RNA sorting
RNA:RNA interactions
germ granules
homotypic RNA assembly
nanos
oskar
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
04 06 2020
04 06 2020
Historique:
received:
14
11
2019
revised:
29
02
2020
accepted:
05
05
2020
pubmed:
29
5
2020
medline:
20
9
2020
entrez:
29
5
2020
Statut:
ppublish
Résumé
mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism by which multiple mRNAs derived from the same gene assemble into discrete homotypic clusters. We demonstrate that in vivo mRNA localization to granules and self-assembly within granules are governed by different mRNA features: localization is encoded by specific RNA regions, whereas self-assembly involves the entire mRNA, does not involve sequence-specific, ordered intermolecular RNA:RNA interactions, and is thus RNA sequence independent. We propose that the ability of mRNAs to self-sort into homotypic assemblies is an inherent property of an messenger ribonucleoprotein (mRNP) that is augmented under conditions that increase RNA concentration, such as upon enrichment in RNA-protein granules, a process that appears conserved in diverse cellular contexts and organisms.
Identifiants
pubmed: 32464092
pii: S1097-2765(20)30309-9
doi: 10.1016/j.molcel.2020.05.008
pmc: PMC7325742
mid: NIHMS1597975
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Nuclear Proteins
0
RNA, Messenger
0
Ribonucleoproteins
0
messenger ribonucleoprotein
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
941-950.e12Subventions
Organisme : NICHD NIH HHS
ID : K99 HD088675
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM126967
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067758
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007388
Pays : United States
Organisme : NICHD NIH HHS
ID : R00 HD088675
Pays : United States
Organisme : NICHD NIH HHS
ID : R37 HD041900
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests H.S. receives royalties, as co-inventor, from sales of iSIM by Visitech. The NIH, its officers, and staff do not recommend or endorse any company, product, or service.
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