Cryopreservation of swine colostrum-derived cells.

Animals Cell Survival Colostrum Cryopreservation / methods Cryoprotective Agents / pharmacology Dimethyl Sulfoxide / pharmacology Female Osteogenesis Pregnancy Staphylococcus aureus Swine
Antibiotics Antimicrobial assay Colostrum Dimethyl sulfoxide Flow cytometry Glycerol Membrane integrity Mesenchymal stromal cell (MSC)-like cells

Journal

Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252

Informations de publication

Date de publication:
12 2020
Historique:
received: 26 02 2020
revised: 19 05 2020
accepted: 21 05 2020
pubmed: 29 5 2020
medline: 27 4 2021
entrez: 29 5 2020
Statut: ppublish

Résumé

Mesenchymal stromal cells (MSCs) have been demonstrated to possess anti-inflammatory and antimicrobial properties and are of interest in biotechnologies that will require cryopreservation. Recently, MSC-like cells were isolated from colostrum and milk. We used an interrupted slow freezing procedure to examine cryoinjury incurred during slow cooling and rapid cooling of MSC-like cells from swine colostrum. Cells were loaded with either dimethyl sulfoxide (Me

Identifiants

DOI: 10.1016/j.cryobiol.2020.05.010 PMID: 32464145
pubmed: 32464145
pii: S0011-2240(20)30079-1
doi: 10.1016/j.cryobiol.2020.05.010
pii:
doi:

Substances chimiques

Cryoprotective Agents 0
Dimethyl Sulfoxide YOW8V9698H

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

168-178

Informations de copyright

Copyright © 2020. Published by Elsevier Inc.

Auteurs

Leah A Marquez-Curtis (LA)

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.

Loredana S Dorobantu (LS)

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.

Dominic Sauvageau (D)

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.

Janet A W Elliott (JAW)

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada. Electronic address: janet.elliott@ualberta.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Survie cellulaire › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Liquides et sécrétions biologiques › Sécrétions corporelles › Colostrum › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Cryoconservation › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Cryoprotecteurs › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Sulfoxydes › Diméthylsulfoxyde › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système locomoteur › Développement locomoteur › Biominéralisation › Développement osseux › Ostéogenèse › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Cryopreservation of swine colostrum-derived cells.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Cryopreservation of swine colostrum-derived cells.