Report on the 19th International Society of Blood Transfusion Platelet Immunology Workshop 2018.
alloantibody detection
anti-HLA antibodies
anti-HPA
genotyping
platelet
platelet antigens
platelet immunology workshop
Journal
Vox sanguinis
ISSN: 1423-0410
Titre abrégé: Vox Sang
Pays: England
ID NLM: 0413606
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
24
02
2020
revised:
30
04
2020
accepted:
05
05
2020
pubmed:
30
5
2020
medline:
16
3
2021
entrez:
30
5
2020
Statut:
ppublish
Résumé
The aims of the 19th International Society of Blood Transfusion Platelet Immunology Workshop were to compare the sensitivity and specificity of in-house and commercially available methods for the detection of alloantibodies against human platelet antigens. Survey regarding laboratory management of samples collected for the diagnosis of foetal neonatal alloimmune thrombocytopenia was also conducted. Twenty-nine laboratories from 17 countries were invited to participate. Seven serum or plasma samples for antibody identification and eight DNA samples for genotyping were sent to participating laboratories. Additionally, samples, critical reagents, materials and instructions for three exercises, one using a commercial kit (Pak Lx), one on platelet preparation for the detection of anti-HPA-3 antibodies and one for testing four anti-CD109 monoclonal antibodies for anti-HPA-15 antibody detection, were provided. Anti-HPA-1a, anti-HPA-2b, anti-HPA-5b and anti-GPIV were detected by the majority of the 28 reporting laboratories using their respective in-house MAIPA assay and/or a commercially available assay. Conversely, very few laboratories correctly identified anti-HPA-3a and HPA-15b. DNA genotyping of HPA and HLA alleles was highly accurate, with just a few discrepancies relative to the expected results. The Pak Lx kit has proven reliable for detecting anti-HPA-1a, anti-HPA-5a and anti-HLA; however, it failed at identifying an anti-HPA-3a in a clinical sample. Some anti-platelet alloantibodies are reliably and consistently detected, yet others remain difficult to detect. Genotyping of HPA and HLA alleles has proven to be highly accurate and robust. Future work should focus on optimizing the detection of anti-HPA-3 and anti-HPA-15 antibodies.
Sections du résumé
BACKGROUND AND OBJECTIVES
OBJECTIVE
The aims of the 19th International Society of Blood Transfusion Platelet Immunology Workshop were to compare the sensitivity and specificity of in-house and commercially available methods for the detection of alloantibodies against human platelet antigens. Survey regarding laboratory management of samples collected for the diagnosis of foetal neonatal alloimmune thrombocytopenia was also conducted.
MATERIALS AND METHODS
METHODS
Twenty-nine laboratories from 17 countries were invited to participate. Seven serum or plasma samples for antibody identification and eight DNA samples for genotyping were sent to participating laboratories. Additionally, samples, critical reagents, materials and instructions for three exercises, one using a commercial kit (Pak Lx), one on platelet preparation for the detection of anti-HPA-3 antibodies and one for testing four anti-CD109 monoclonal antibodies for anti-HPA-15 antibody detection, were provided.
RESULTS
RESULTS
Anti-HPA-1a, anti-HPA-2b, anti-HPA-5b and anti-GPIV were detected by the majority of the 28 reporting laboratories using their respective in-house MAIPA assay and/or a commercially available assay. Conversely, very few laboratories correctly identified anti-HPA-3a and HPA-15b. DNA genotyping of HPA and HLA alleles was highly accurate, with just a few discrepancies relative to the expected results. The Pak Lx kit has proven reliable for detecting anti-HPA-1a, anti-HPA-5a and anti-HLA; however, it failed at identifying an anti-HPA-3a in a clinical sample.
CONCLUSIONS
CONCLUSIONS
Some anti-platelet alloantibodies are reliably and consistently detected, yet others remain difficult to detect. Genotyping of HPA and HLA alleles has proven to be highly accurate and robust. Future work should focus on optimizing the detection of anti-HPA-3 and anti-HPA-15 antibodies.
Substances chimiques
Antigens, Human Platelet
0
ITGB3 protein, human
0
Integrin beta3
0
Isoantibodies
0
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
767-782Informations de copyright
© 2020 International Society of Blood Transfusion.
Références
IATA. IATA Dangerous Goods Regulations, 58th Edition, Packing Instructions 650 [Internet]. IATA; 2017 [cited 2019 Oct 16]. Available from: https://www.iata.org/whatwedo/cargo/dgr/Documents/packing-instruction-650-DGR56-en.pdf.
Bakchoul T, Kubiak S, Krautwurst A, et al.: Low-avidity anti-HPA-1a alloantibodies are capable of antigen-positive platelet destruction in the NOD/SCID mouse model of alloimmune thrombocytopenia. Transfusion (Paris) 2011; 51(11):2455-61
Delbos F, Bertrand G, Croisille L, et al.: Fetal and neonatal alloimmune thrombocytopenia: predictive factors of intracranial hemorrhage. Transfusion (Paris) 2016; 56(1):59-66; quiz 58
Sonneveld ME, Natunen S, Sainio S, et al.: Glycosylation pattern of anti-platelet IgG is stable during pregnancy and predicts clinical outcome in alloimmune thrombocytopenia. Br J Haematol 2016; 174(2):310-20
Kapur R, Kustiawan I, Vestrheim A, et al.: A prominent lack of IgG1-Fc fucosylation of platelet alloantibodies in pregnancy. Blood 2014; 123(4):471-80
Sachs UJ: Prospects for risk stratification of anti-HPA-1a alloimmunized pregnant women. Transfus Apher Sci 2019; 31:102709
Wikman A, Mörtberg A, Sachs UJ, et al.: Report on the 18th Platelet Immunology Workshop of the ISBT 2016. ISBT Sci Ser 2017; 12(1):214-22
Bertrand G, Bakchoul T, Javela K, et al.: SSC of the ISTH. Interlaboratory workshop on anti-HPA-1a alloantibody quantification with the mAb-specific immobilization of platelet antigen technique. J Thromb Haemost 2012; 10(6):1172-4
Li RS, Ling B, Lu P: Development of quantitative monoclonal antibody-specific immobilization of platelet antigens assay for antibodies against human platelet antigen-1a, 3a, and 5b. Platelets 2018; 29(1):71-5
Tao S, Chen S, Hong X, et al.: Novel method for simultaneously detecting HPA and HLA antibodies using Luminex microbeads. J Transl Med 2019; 17(1):249
Metzner K, Bauer J, Ponzi H, et al.: Detection and identification of platelet antibodies using a sensitive multiplex assay system-platelet antibody bead array. Transfusion (Paris) 2017; 57(7):1724-33
Mörtberg A, Meinke S, Berg P, et al.: Sensitive detection of platelet-specific antibodies with a modified MAIPA using biotinylated antibodies and streptavidin-coated beads. J Immunol Methods 2016; 434:9-15
Maślanka K, Michur H, Guz K, et al.: The relevance of HPA-15 antigen expression for anti-HPA-15 antibody detection. Int J Lab Hematol 2012; 34(1):65-9