Functional characterization of neuropeptide 26RFa receptors GPR103A and GPR103B in zebrafish, Danio rerio.
26RFa
G-protein-coupled receptor (GPCR)
Neuropeptide
Signal transduction
Zebrafish
Journal
Cellular signalling
ISSN: 1873-3913
Titre abrégé: Cell Signal
Pays: England
ID NLM: 8904683
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
26
11
2019
revised:
10
05
2020
accepted:
23
05
2020
pubmed:
30
5
2020
medline:
26
10
2021
entrez:
30
5
2020
Statut:
ppublish
Résumé
The hypothalamic neuropeptide 26RFa is the most recently identified member of the RFamide peptide family, and this 26RFa signaling system has been shown to be implicated in regulating a variety of physiological processes. In zebrafish，26RFa and two putative receptors, DrGPR103A and DrGPR103B, have been in silico identified, and in vivo data derived from overexpression and loss of function mutation experiments suggest the 26RFa signaling system plays an important role in the hypothalamic regulation of sleep. However, the biochemical and pharmacological information on DrGPR103A/B receptors is still unknown. Here, after cloning of cDNAs of two putative 26RFa receptor genes, DrGPR103A and B, from the total RNA of zebrafish whole body, functional assays demonstrated that both receptors were activated by synthetic zebrafish 26RFa neuropeptide, leading to a significant increase in CRE-driven luciferase activity and intracellular Ca
Identifiants
pubmed: 32470519
pii: S0898-6568(20)30154-6
doi: 10.1016/j.cellsig.2020.109677
pii:
doi:
Substances chimiques
Neuropeptides
0
Receptors, G-Protein-Coupled
0
prepro-26RFa protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
109677Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.