Novel dot-blot assay for detection of vascular Notch3 aggregates in patients with CADASIL.
Biopsy
CADASIL
Diagnosis
Dot-blot assay
Notch3
Journal
Journal of the neurological sciences
ISSN: 1878-5883
Titre abrégé: J Neurol Sci
Pays: Netherlands
ID NLM: 0375403
Informations de publication
Date de publication:
15 08 2020
15 08 2020
Historique:
received:
23
11
2019
revised:
05
05
2020
accepted:
17
05
2020
pubmed:
30
5
2020
medline:
15
5
2021
entrez:
30
5
2020
Statut:
ppublish
Résumé
To detect vascular Notch3 extracellular domain aggregates in CADASIL, we developed a novel dot-blot assay with both autopsy and biopsy skin samples. We obtained samples from 11 patients with CADASIL and 12 control patients, and we performed dot-blot analyses by using sequential biochemical tissue extractions with three different antibodies against specific regions of the Notch3 extracellular domain. We also analyzed clinical features and vascular accumulations of Notch3 by immunohistochemistry. Via the dot-blot assay with the antibody against the C-terminal region of the Notch3 extracellular domain, we successfully detected Notch3 extracellular domain aggregates in skin tissue homogenates obtained from patients with CADASIL. Our novel method may therefore aid the diagnosis of CADASIL.
Identifiants
pubmed: 32470649
pii: S0022-510X(20)30268-9
doi: 10.1016/j.jns.2020.116931
pii:
doi:
Substances chimiques
NOTCH3 protein, human
0
Receptor, Notch3
0
Receptors, Notch
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116931Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest All authors declare no conflict of interests.