Identification and analysis of key lncRNAs in malignant-transformed BEAS-2B cells induced with coal tar pitch by microarray analysis.
BEAS-2B cells
CTPE
Long-non-coding RNA
Lung cancer
microarray analysis
Journal
Environmental toxicology and pharmacology
ISSN: 1872-7077
Titre abrégé: Environ Toxicol Pharmacol
Pays: Netherlands
ID NLM: 9612020
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
26
06
2019
revised:
23
03
2020
accepted:
23
03
2020
pubmed:
30
5
2020
medline:
5
2
2021
entrez:
30
5
2020
Statut:
ppublish
Résumé
This study aims to explore the key and differentially expressed long non-coding RNAs (lncRNAs) and elucidates their possible mechanisms in malignant-transformed Human bronchial epithelial (BEAS-2B) cells induced by coal tar pitch extracts (CTPE). BEAS-2B cells were stimulated with 2.4 μg/ml CTPE, then passaged for three times which were named CTPE1 and then passaged until passage 30 (CTPE30). The results showed that cells of CTPE30 appeared abnormal morphology. Furthermore, migration, clonality and proliferation of cells in CTPE group were significantly increased compared with those in control groups. However, the apoptosis of cells in CTPE group was inhibited. A total of 569 differentially expressed mRNAs and 707 differentially expressed lncRNAs were screened out, among which four lncRNAs were validated and were consistent with the microarray results. 32 target genes were screened out by Co-expression network. The study suggests that differentially expressed lncRNAs may play a potential role in lung carcinogenesis.
Identifiants
pubmed: 32470693
pii: S1382-6689(20)30052-1
doi: 10.1016/j.etap.2020.103376
pii:
doi:
Substances chimiques
RNA, Long Noncoding
0
RNA, Messenger
0
Coal Tar
8007-45-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103376Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.