Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture.
DNA aptamer
cell culture
mycoplasma
rapid detection
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 May 2020
27 May 2020
Historique:
received:
28
04
2020
revised:
21
05
2020
accepted:
25
05
2020
entrez:
31
5
2020
pubmed:
31
5
2020
medline:
13
2
2021
Statut:
epublish
Résumé
Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive.
Identifiants
pubmed: 32471128
pii: ijms21113784
doi: 10.3390/ijms21113784
pmc: PMC7312096
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R01 CA224304
Pays : United States
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