The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.
cell size
embryonic development
nuclear size scaling
nuclear-to-cytoplasmic ratio
nucleus
paracentrotus lividus sea urchins
perinuclear endoplasmic reticulum
xenopus laevis frogs
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
10 08 2020
10 08 2020
Historique:
received:
08
10
2019
revised:
26
03
2020
accepted:
05
05
2020
pubmed:
31
5
2020
medline:
26
1
2021
entrez:
31
5
2020
Statut:
ppublish
Résumé
Nuclear size plays pivotal roles in gene expression, embryo development, and disease. A central hypothesis in organisms ranging from yeast to vertebrates is that nuclear size scales to cell size. This implies that nuclei may reach steady-state sizes set by limiting cytoplasmic pools of size-regulating components. By monitoring nuclear dynamics in early sea urchin embryos, we found that nuclei undergo substantial growth in each interphase, reaching a maximal size prior to mitosis that declined steadily over the course of development. Manipulations of cytoplasmic volume through multiple chemical and physical means ruled out cell size as a major determinant of nuclear size and growth. Rather, our data suggest that the perinuclear endoplasmic reticulum, accumulated through dynein activity, serves as a limiting membrane pool that sets nuclear surface growth rate. Partitioning of this local pool at each cell division modulates nuclear growth kinetics and dictates size scaling throughout early development.
Identifiants
pubmed: 32473090
pii: S1534-5807(20)30359-2
doi: 10.1016/j.devcel.2020.05.003
pmc: PMC7423768
mid: NIHMS1593923
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
395-409.e7Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103432
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM113028
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134885
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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