Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis.
Adaptor Proteins, Signal Transducing
/ genetics
Animals
Carcinogenesis
/ genetics
Cell Line, Tumor
Cell Proliferation
Colorectal Neoplasms
/ genetics
Cytoskeletal Proteins
/ genetics
Female
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
LIM Domain Proteins
/ genetics
Male
Mice, Inbred BALB C
Mice, Nude
MicroRNAs
/ genetics
Middle Aged
RNA, Circular
/ genetics
Up-Regulation
Circ-FARSA
Colorectal cancer
LIM and SH3 protein 1
miR-330-5p
Journal
Archives of biochemistry and biophysics
ISSN: 1096-0384
Titre abrégé: Arch Biochem Biophys
Pays: United States
ID NLM: 0372430
Informations de publication
Date de publication:
15 08 2020
15 08 2020
Historique:
received:
14
01
2020
revised:
21
05
2020
accepted:
22
05
2020
pubmed:
1
6
2020
medline:
10
10
2020
entrez:
1
6
2020
Statut:
ppublish
Résumé
Circular RNA (circRNA) has been proposed to be involved in carcinogenesis. Here, we explored the functional significance and regulatory role of circ-FARSA in colorectal cancer (CRC). Gene expression was determined using quantitative reverse transcriptase polymerase chain reaction and Western blot. We determined the effect of circFARSA on CRC progression using cell count kit-8, colony formation assay, wound-healing assay, transwell invasion assay, luciferase reporter assay and in vivo assay. circ-FARSA was upregulated in CRC tissues and cell lines, and its expression had a significant association with the overall survival of CRC patients. Knockdown of circ-FARSA inhibited the proliferation, migration, and invasion of CRC cells in vitro. Moreover, circ-FARSA functioned as a sponge of miR-330-5p, and its upregulation mitigated the inhibitory effects of miR-330-5p on CRC cell proliferation and metastasis. In addition, circ-FARSA regulated the expression of LIM and SH3 protein 1 (LASP1) by sponging miR-330-5p. Besides, inhibition of circ-FARSA repressed the growth of CRC in vivo. Silencing of circ-FARSA restricted the growth of CRC through regulating the miR-330-5p/LASP1 axis, providing a novel regulatory mechanism for CRC tumorigenesis.
Sections du résumé
BACKGROUND
Circular RNA (circRNA) has been proposed to be involved in carcinogenesis. Here, we explored the functional significance and regulatory role of circ-FARSA in colorectal cancer (CRC).
METHODS
Gene expression was determined using quantitative reverse transcriptase polymerase chain reaction and Western blot. We determined the effect of circFARSA on CRC progression using cell count kit-8, colony formation assay, wound-healing assay, transwell invasion assay, luciferase reporter assay and in vivo assay.
RESULT
circ-FARSA was upregulated in CRC tissues and cell lines, and its expression had a significant association with the overall survival of CRC patients. Knockdown of circ-FARSA inhibited the proliferation, migration, and invasion of CRC cells in vitro. Moreover, circ-FARSA functioned as a sponge of miR-330-5p, and its upregulation mitigated the inhibitory effects of miR-330-5p on CRC cell proliferation and metastasis. In addition, circ-FARSA regulated the expression of LIM and SH3 protein 1 (LASP1) by sponging miR-330-5p. Besides, inhibition of circ-FARSA repressed the growth of CRC in vivo.
CONCLUSION
Silencing of circ-FARSA restricted the growth of CRC through regulating the miR-330-5p/LASP1 axis, providing a novel regulatory mechanism for CRC tumorigenesis.
Identifiants
pubmed: 32473899
pii: S0003-9861(20)30443-4
doi: 10.1016/j.abb.2020.108434
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Cytoskeletal Proteins
0
LASP1 protein, human
0
LIM Domain Proteins
0
MIRN330 microRNA, human
0
MicroRNAs
0
RNA, Circular
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108434Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no competing interests.