Instability of urinary excreted methyl-2-acetamido-2-deoxy-1-seleno-β-d-galactopyranoside (selenosugar 1), the main elimination product of human selenium metabolism, and measures for its stabilization.
Methylselenic acid
Selenium
Selenosugar 1
Stability
Urine
Journal
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)
ISSN: 1878-3252
Titre abrégé: J Trace Elem Med Biol
Pays: Germany
ID NLM: 9508274
Informations de publication
Date de publication:
05 May 2020
05 May 2020
Historique:
received:
06
03
2020
revised:
14
04
2020
accepted:
21
04
2020
pubmed:
1
6
2020
medline:
1
6
2020
entrez:
1
6
2020
Statut:
aheadofprint
Résumé
The urinary excreted selenium species selenosugar 1 (SeSug1) plays a key role for monitoring of supplemental selenium exposure, e.g. by occupational exposure. In order to reproduce its contents in the long term, the integrity of SeSug1 in the urine is essential. Studies on the stability of SeSug1 in urine samples stored at -20 °C have shown that degradation of SeSug 1 occurs, requiring adequate countermeasures. Here, we explored the long-term stability of SeSug1 under usual storage conditions at -20 °C. For this purpose, the simultaneous determination of selenosugar 1 and methylselenic acid (MeSeA) was used to explore the stabilizing of the SeSug1 content by applying sodium azide (NaN In untreated urine, conversion of SeSug1 to MeSeA was evident within days. Differences in urine matrices clearly showed different impact, which could be attributed to different buffer strengths by the urine itself. For durability, various concentrations of sodium azide were first applied, followed by pH buffering. A combination of 0.1% NaN The formation of MeSeA as degradation product of SeSug1 could be confirmed. Based on the proportions, an oxidation-based decomposition pathway was proposed. The investigations revealed that the complex interaction of pH buffering and bactericidal activity must be taken into account in order to stabilize SeSug1 in the urine. The main effect was the addition of NaN
Sections du résumé
BACKGROUND
BACKGROUND
The urinary excreted selenium species selenosugar 1 (SeSug1) plays a key role for monitoring of supplemental selenium exposure, e.g. by occupational exposure. In order to reproduce its contents in the long term, the integrity of SeSug1 in the urine is essential. Studies on the stability of SeSug1 in urine samples stored at -20 °C have shown that degradation of SeSug 1 occurs, requiring adequate countermeasures.
METHODS
METHODS
Here, we explored the long-term stability of SeSug1 under usual storage conditions at -20 °C. For this purpose, the simultaneous determination of selenosugar 1 and methylselenic acid (MeSeA) was used to explore the stabilizing of the SeSug1 content by applying sodium azide (NaN
RESULTS
RESULTS
In untreated urine, conversion of SeSug1 to MeSeA was evident within days. Differences in urine matrices clearly showed different impact, which could be attributed to different buffer strengths by the urine itself. For durability, various concentrations of sodium azide were first applied, followed by pH buffering. A combination of 0.1% NaN
CONCLUSION
CONCLUSIONS
The formation of MeSeA as degradation product of SeSug1 could be confirmed. Based on the proportions, an oxidation-based decomposition pathway was proposed. The investigations revealed that the complex interaction of pH buffering and bactericidal activity must be taken into account in order to stabilize SeSug1 in the urine. The main effect was the addition of NaN
Identifiants
pubmed: 32474349
pii: S0946-672X(20)30103-6
doi: 10.1016/j.jtemb.2020.126538
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126538Informations de copyright
Copyright © 2020 Elsevier GmbH. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that there is no conflict of interest