Using a PCR instrument to hydrolyze polysaccharides for monosaccharide composition analyses.
Method development
Monosaccharide composition
PCR instrument
Polysaccharide hydrolysis
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
15 Jul 2020
15 Jul 2020
Historique:
received:
04
02
2020
revised:
03
04
2020
accepted:
16
04
2020
entrez:
2
6
2020
pubmed:
2
6
2020
medline:
12
1
2021
Statut:
ppublish
Résumé
Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides. To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min. Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (3
Identifiants
pubmed: 32475593
pii: S0144-8617(20)30512-9
doi: 10.1016/j.carbpol.2020.116338
pii:
doi:
Substances chimiques
Monosaccharides
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116338Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no conflicts of interest with content of the manuscript.