Phasor approach to autofluorescence lifetime imaging FLIM can be a quantitative biomarker of chronic renal parenchymal injury.
FLIM
NADH
autofluorescence
phasor
tubulointerstitial fibrosis
Journal
Kidney international
ISSN: 1523-1755
Titre abrégé: Kidney Int
Pays: United States
ID NLM: 0323470
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
10
12
2019
revised:
21
01
2020
accepted:
13
02
2020
pubmed:
2
6
2020
medline:
22
6
2021
entrez:
2
6
2020
Statut:
ppublish
Résumé
Diabetic kidney disease continues to be the leading cause of chronic kidney disease, often advancing to end stage kidney disease. In addition to the well characterized glomerular alterations including mesangial expansion, podocyte injury, and glomerulosclerosis, tubulointerstitial fibrosis is also an important component of diabetic kidney injury. Similarly, tubulointerstitial fibrosis is a critical component of any chronic kidney injury. Therefore, sensitive and quantitative identification of tubulointerstitial fibrosis is critical for the assessment of long-term prognosis of kidney disease. Here, we employed phasor approach to fluorescence lifetime imaging, commonly known as FLIM, to understand tissue heterogeneity and calculate changes in the tissue autofluorescence lifetime signatures due to diabetic kidney disease. FLIM imaging was performed on cryostat sections of snap-frozen biopsy material of patients with diabetic nephropathy. There was an overall increase in phase lifetime (τ
Identifiants
pubmed: 32475606
pii: S0085-2538(20)30261-1
doi: 10.1016/j.kint.2020.02.019
pmc: PMC7606347
mid: NIHMS1598407
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1341-1346Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK062209
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG049493
Pays : United States
Organisme : BLRD VA
ID : I01 BX001954
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK098336
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK116567
Pays : United States
Organisme : NIGMS NIH HHS
ID : P50 GM076516
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103540
Pays : United States
Informations de copyright
Copyright © 2020 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.
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