Mesenchymal stem cells from orthodontic premolar teeth.
Bone marrow mesenchymal stem cells
Dental pulp mesenchymal stem cells
Orthodontic premolar teeth
Journal
Medical journal, Armed Forces India
ISSN: 0377-1237
Titre abrégé: Med J Armed Forces India
Pays: India
ID NLM: 7602492
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
25
02
2017
accepted:
08
08
2018
entrez:
2
6
2020
pubmed:
2
6
2020
medline:
2
6
2020
Statut:
ppublish
Résumé
Considering the limitations in isolating Bone Marrow Mesenchymal Stem Cells (BMSCs), alternate sources of Mesenchymal Stem Cells (MSCs) are being intensely investigated. This study evaluated dental pulp MSCs (DP-MSCs) isolated from orthodontically extracted premolar teeth from a bone tissue engineering perspective. MSCs isolated from premolar teeth pulp were cultured and studied using BMSCs as the control. Flow cytometry analysis was performed for the positive and negative MSC markers. Multilineage differentiation focusing on bone regeneration was evaluated by specific growth induction culturing media and by alkaline phosphatase (ALP) activity. Data were compared by repeated measurement analysis of variance and Student's t-test at a Proliferation rate, population doubling time, and colony formation of DP-MSCs were significantly higher (p < 0.001) than BMSCs. More than 85% of DP-MSCs expressed CD44, CD73, CD90, CD105, and CD166. Negative reaction was found for CD11b CD33, CD34, and CD45. Positive reaction was displayed by 7.2% of cells for early MSC marker, Stro-1. Both the cell populations differentiated into adipogenic, osteogenic, and chondrogenic lineages, with adequate ALP expression. Because DP-MSCs from orthodontic premolars hold a neural crest/ectomesenchymal ancestry, its prudent growth characteristics and multilineage differentiation open up exciting options in craniofacial tissue engineering.
Sections du résumé
BACKGROUND
BACKGROUND
Considering the limitations in isolating Bone Marrow Mesenchymal Stem Cells (BMSCs), alternate sources of Mesenchymal Stem Cells (MSCs) are being intensely investigated. This study evaluated dental pulp MSCs (DP-MSCs) isolated from orthodontically extracted premolar teeth from a bone tissue engineering perspective.
METHODS
METHODS
MSCs isolated from premolar teeth pulp were cultured and studied using BMSCs as the control. Flow cytometry analysis was performed for the positive and negative MSC markers. Multilineage differentiation focusing on bone regeneration was evaluated by specific growth induction culturing media and by alkaline phosphatase (ALP) activity. Data were compared by repeated measurement analysis of variance and Student's t-test at a
RESULTS
RESULTS
Proliferation rate, population doubling time, and colony formation of DP-MSCs were significantly higher (p < 0.001) than BMSCs. More than 85% of DP-MSCs expressed CD44, CD73, CD90, CD105, and CD166. Negative reaction was found for CD11b CD33, CD34, and CD45. Positive reaction was displayed by 7.2% of cells for early MSC marker, Stro-1. Both the cell populations differentiated into adipogenic, osteogenic, and chondrogenic lineages, with adequate ALP expression.
CONCLUSION
CONCLUSIONS
Because DP-MSCs from orthodontic premolars hold a neural crest/ectomesenchymal ancestry, its prudent growth characteristics and multilineage differentiation open up exciting options in craniofacial tissue engineering.
Identifiants
pubmed: 32476715
doi: 10.1016/j.mjafi.2018.08.006
pii: S0377-1237(18)30114-X
pmc: PMC7244874
doi:
Types de publication
Journal Article
Langues
eng
Pagination
172-179Informations de copyright
© 2018 Director General, Armed Forces Medical Services. Published by Elsevier, a division of RELX India Pvt. Ltd.
Références
J Oral Pathol Med. 2008 Oct;37(9):571-4
pubmed: 18331285
Acta Pharmacol Sin. 2013 Jun;34(6):747-54
pubmed: 23736003
Tissue Eng Part C Methods. 2008 Jun;14(2):149-56
pubmed: 18489245
Eur Cell Mater. 2012 Jan 12;23:13-27
pubmed: 22241610
J Dent (Tehran). 2010 Fall;7(4):185-95
pubmed: 21998794
Cytotherapy. 2006;8(4):315-7
pubmed: 16923606
J Dent Res. 2015 Nov;94(11):1495-501
pubmed: 26350960
Stem Cells. 2008 Sep;26(9):2287-99
pubmed: 18566331
Stem Cells. 2017 Jan;35(1):61-67
pubmed: 27273755
J Prosthodont Res. 2012 Jul;56(3):151-65
pubmed: 22796367
J Endod. 2008 Feb;34(2):166-71
pubmed: 18215674
Lancet. 2004 Jul 10-16;364(9429):149-55
pubmed: 15246727
Eur Cell Mater. 2011 Jul 06;22:26-42
pubmed: 21732280
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5807-12
pubmed: 12716973
Indian J Dent Res. 2014 Jan-Feb;25(1):14-21
pubmed: 24748292
Matrix Biol. 2005 Apr;24(2):155-65
pubmed: 15890265
Dev Dyn. 2008 Mar;237(3):592-601
pubmed: 18224711
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13625-30
pubmed: 11087820
Arthritis Res Ther. 2007;9(1):204
pubmed: 17316462
Histochem Cell Biol. 2010 Jan;133(1):95-112
pubmed: 19816704
Stem Cells. 2010 Mar 31;28(3):585-96
pubmed: 19967788
BMC Dev Biol. 2015 Mar 14;15:14
pubmed: 25879198
Bone Marrow Res. 2011;2011:353878
pubmed: 22046560