Folic acid attenuates high-fat diet-induced steatohepatitis
Animals
Cell Line, Tumor
DNA, Bacterial
/ isolation & purification
Diet, High-Fat
/ adverse effects
Disease Models, Animal
Feces
/ microbiology
Folic Acid
/ pharmacology
Gastrointestinal Microbiome
/ drug effects
Humans
Lipid Metabolism
/ drug effects
Liver
/ drug effects
Male
Non-alcoholic Fatty Liver Disease
/ drug therapy
PPAR alpha
/ metabolism
RNA, Ribosomal, 16S
/ genetics
RNA, Small Interfering
/ metabolism
Rats
Sirtuin 1
/ genetics
Up-Regulation
/ drug effects
Folic acid
Gut microbiota
Nonalcoholic fatty liver disease
PPARα
SIRT1
Journal
World journal of gastroenterology
ISSN: 2219-2840
Titre abrégé: World J Gastroenterol
Pays: United States
ID NLM: 100883448
Informations de publication
Date de publication:
14 May 2020
14 May 2020
Historique:
received:
31
12
2019
revised:
27
03
2020
accepted:
17
04
2020
entrez:
2
6
2020
pubmed:
2
6
2020
medline:
9
2
2021
Statut:
ppublish
Résumé
Folic acid has been shown to improve non-alcoholic steatohepatitis (NASH), but its roles in hepatic lipid metabolism, hepatic one-carbon metabolism, and gut microbiota are still unknown. To demonstrate the role of folic acid in lipid metabolism and gut microbiota in NASH. Twenty-four Sprague-Dawley rats were assigned into three groups: Chow diet, high-fat diet (HFD), and HFD with folic acid administration. At the end of 16 wk, the liver histology, the expression of hepatic genes related to lipid metabolism, one-carbon metabolism, and gut microbiota structure analysis of fecal samples based on 16S rRNA sequencing were measured to evaluate the effect of folic acid. Palmitic acid-exposed Huh7 cell line was used to evaluate the role of folic acid in hepatic lipid metabolism. Folic acid treatment attenuated steatosis, lobular inflammation, and hepatocellular ballooning in rats with HFD-induced steatohepatitis. Genes related to lipid Folic acid improves hepatic lipid metabolism by upregulating PPARα levels
Sections du résumé
BACKGROUND
BACKGROUND
Folic acid has been shown to improve non-alcoholic steatohepatitis (NASH), but its roles in hepatic lipid metabolism, hepatic one-carbon metabolism, and gut microbiota are still unknown.
AIM
OBJECTIVE
To demonstrate the role of folic acid in lipid metabolism and gut microbiota in NASH.
METHODS
METHODS
Twenty-four Sprague-Dawley rats were assigned into three groups: Chow diet, high-fat diet (HFD), and HFD with folic acid administration. At the end of 16 wk, the liver histology, the expression of hepatic genes related to lipid metabolism, one-carbon metabolism, and gut microbiota structure analysis of fecal samples based on 16S rRNA sequencing were measured to evaluate the effect of folic acid. Palmitic acid-exposed Huh7 cell line was used to evaluate the role of folic acid in hepatic lipid metabolism.
RESULTS
RESULTS
Folic acid treatment attenuated steatosis, lobular inflammation, and hepatocellular ballooning in rats with HFD-induced steatohepatitis. Genes related to lipid
CONCLUSION
CONCLUSIONS
Folic acid improves hepatic lipid metabolism by upregulating PPARα levels
Identifiants
pubmed: 32476787
doi: 10.3748/wjg.v26.i18.2203
pmc: PMC7235203
doi:
Substances chimiques
DNA, Bacterial
0
PPAR alpha
0
RNA, Ribosomal, 16S
0
RNA, Small Interfering
0
Folic Acid
935E97BOY8
Sirtuin 1
EC 3.5.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2203-2220Informations de copyright
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict-of-interest statement: The authors declare that there is no conflict of interest related to this study.
Références
Cell Metab. 2009 Apr;9(4):327-38
pubmed: 19356714
Exp Mol Med. 2019 Sep 10;51(9):1-14
pubmed: 31506421
J Hepatol. 2017 Oct;67(4):862-873
pubmed: 28642059
Nutrients. 2018 Apr 02;10(4):
pubmed: 29614799
Nutrition. 2005 Feb;21(2):137-41
pubmed: 15723740
Genes Cancer. 2011 Feb;2(2):130-9
pubmed: 21779486
Eur J Pharmacol. 2019 Mar 15;847:72-82
pubmed: 30689994
Nature. 2000 May 25;405(6785):421-4
pubmed: 10839530
Aliment Pharmacol Ther. 2018 Jun;47(12):1639-1651
pubmed: 29696666
Hepatology. 2010 Dec;52(6):1992-2000
pubmed: 20890895
Nature. 2006 Dec 14;444(7121):868-74
pubmed: 17167475
Nutr Res Pract. 2011 Dec;5(6):520-6
pubmed: 22259676
J Hepatol. 2019 Mar;70(3):531-544
pubmed: 30414863
Alcohol Alcohol. 2016 May;51(3):235-41
pubmed: 26433946
Can J Physiol Pharmacol. 2017 Oct;95(10):1141-1148
pubmed: 28460180
Am J Physiol Regul Integr Comp Physiol. 2015 Nov 15;309(10):R1215-25
pubmed: 26400185
Cell Metab. 2013 Aug 6;18(2):296-302
pubmed: 23931760
Oxid Med Cell Longev. 2019 Jun 11;2019:1342549
pubmed: 31308875
Clin Epigenetics. 2018 Jul 13;10(1):93
pubmed: 30005700
World J Gastroenterol. 2017 Jan 7;23(1):60-75
pubmed: 28104981
Biofactors. 2014 May-Jun;40(3):277-83
pubmed: 24353111
Pol J Pathol. 2015 Mar;66(1):49-56
pubmed: 26017880
J Dev Orig Health Dis. 2014 Oct;5(5):385-95
pubmed: 25084068
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7473-8
pubmed: 10377439
Hepatology. 2011 Dec;54(6):1975-86
pubmed: 21837751
Hepatology. 2019 Aug;70(2):711-724
pubmed: 30556145
Annu Rev Physiol. 2014;76:251-74
pubmed: 24512081
Hepatology. 2019 Oct;70(4):1095-1098
pubmed: 31298746
J Clin Invest. 2018 Dec 3;128(12):5587-5602
pubmed: 30300140
J Endocrinol. 2017 Jul;234(1):R1-R21
pubmed: 28428362
Biol Rev Camb Philos Soc. 2016 May;91(2):452-68
pubmed: 25740151
J Agric Food Chem. 2018 Dec 19;66(50):13162-13172
pubmed: 30484310
Br J Nutr. 2017 Oct;118(8):580-588
pubmed: 29056104
Gastroenterology. 2013 Nov;145(5):1076-87
pubmed: 23916847
Environ Toxicol. 2012 May;27(6):351-63
pubmed: 22566245
Congenit Anom (Kyoto). 2017 Sep;57(5):138-141
pubmed: 28603928
Nat Rev Drug Discov. 2016 Apr;15(4):249-74
pubmed: 26794269
J Biol Chem. 1999 Jul 2;274(27):19228-36
pubmed: 10383430
Clin Nutr. 2018 Oct;37(5):1752-1758
pubmed: 28705466
World J Gastroenterol. 2018 Aug 14;24(30):3361-3373
pubmed: 30122876
Int J Food Sci Nutr. 2012 Sep;63(6):659-66
pubmed: 22229957
Gut. 2016 Jul;65(7):1202-14
pubmed: 26838599
World J Gastroenterol. 2018 Jul 21;24(27):2974-2983
pubmed: 30038464
PPAR Res. 2010;2010:
pubmed: 20936127
PLoS One. 2018 Aug 28;13(8):e0202910
pubmed: 30153273
Nutr Metab (Lond). 2013 Feb 03;10(1):20
pubmed: 23374533
Hepatology. 2012 Nov;56(5):1751-9
pubmed: 22707395
Front Endocrinol (Lausanne). 2019 Sep 06;10:611
pubmed: 31555219
Hepatology. 2019 Oct;70(4):1119-1133
pubmed: 31070259
J Nutr Biochem. 2018 Sep;59:76-83
pubmed: 29986310
Int J Mol Sci. 2016 Jul 29;17(8):
pubmed: 27483246
Biochem Biophys Res Commun. 2002 Jan 25;290(3):1114-22
pubmed: 11798191
Lipids. 2018 Jul;53(7):709-716
pubmed: 30252139