Protein Kinase Cɛ in the Platelet and Hippocampal Tissue as a Diagnostic Biological Marker in Alzheimer Disease.
Alzheimer disease
Amyloid Beta
Hippocampus
PKCɛ
Platelet
Journal
Basic and clinical neuroscience
ISSN: 2008-126X
Titre abrégé: Basic Clin Neurosci
Pays: Iran
ID NLM: 101575211
Informations de publication
Date de publication:
Historique:
received:
03
12
2018
revised:
25
01
2019
accepted:
07
02
2019
entrez:
2
6
2020
pubmed:
2
6
2020
medline:
2
6
2020
Statut:
ppublish
Résumé
Alzheimer Disease (AD) is a neurodegenerative disorder characterized by the progressive loss of memory and other cognitive functions. Protein Kinase Cɛ (PKCɛ) is an isoform that most effectively suppresses Amyloid Beta (Aβ) production and synaptic loss. In this study, spatial learning and memory for treated rats were evaluated by the Morris water maze test. The activity (total PKC), mRNA expression, and protein level of PKCɛ in the platelet and hippocampal tissue were evaluated using immunosorbent assay, real-time qPCR, and western blotting analysis, respectively. The traveled distance was significantly prolonged, and escape latency significantly increased in Aβ-treated groups. PKC activity assay showed that there was a remarkable difference between the Aβ-treated and sham-operated groups on days 10 and 30 in the hippocampus and also day 30 in platelet after the injection of Aβ. A significant effect in PKC activity was observed between days 0 and 10, days 0 and 30, as well as days 5 and 30. Aβ significantly downregulated the PKCɛ mRNA expression in the hippocampus of rats on day 30; however, no significant difference was observed in platelet. Western blot analysis demonstrated that Aβ significantly reduced PKCɛ protein expression in the hippocampus of treated groups on day 30. The expression level of PKCɛ was downregulated following the injection of Aβ in the hippocampus, but no significant difference was observed between the AD and sham groups in platelet that may be due to the low concentration of PKCɛ or duration of Aβ exposure in the rat brain.
Identifiants
pubmed: 32477472
doi: 10.32598/bcn.9.10.80.1
pii: BCN-10-545
pmc: PMC7253800
doi:
Types de publication
Journal Article
Langues
eng
Pagination
545-556Informations de copyright
Copyright© 2019 Iranian Neuroscience Society.
Déclaration de conflit d'intérêts
Conflict of interest The authors declared no conflict of interests.
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