Protrudin-mediated ER-endosome contact sites promote MT1-MMP exocytosis and cell invasion.
Breast Neoplasms
/ enzymology
Cell Line, Tumor
Cell Movement
Endoplasmic Reticulum
/ enzymology
Endosomes
/ enzymology
Exocytosis
Extracellular Matrix
/ enzymology
Female
Gene Expression Regulation, Neoplastic
Humans
Matrix Metalloproteinase 14
/ genetics
Microtubule-Associated Proteins
/ genetics
Neoplasm Invasiveness
Podosomes
/ enzymology
Protein Transport
Signal Transduction
Synaptotagmins
/ genetics
Vesicular Transport Proteins
/ genetics
rab GTP-Binding Proteins
/ genetics
rab7 GTP-Binding Proteins
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 08 2020
03 08 2020
Historique:
received:
10
03
2020
revised:
04
05
2020
accepted:
05
05
2020
entrez:
2
6
2020
pubmed:
2
6
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
Cancer cells break tissue barriers by use of small actin-rich membrane protrusions called invadopodia. Complete invadopodia maturation depends on protrusion outgrowth and the targeted delivery of the matrix metalloproteinase MT1-MMP via endosomal transport by mechanisms that are not known. Here, we show that the ER protein Protrudin orchestrates invadopodia maturation and function. Protrudin formed contact sites with MT1-MMP-positive endosomes that contained the RAB7-binding Kinesin-1 adaptor FYCO1, and depletion of RAB7, FYCO1, or Protrudin inhibited MT1-MMP-dependent extracellular matrix degradation and cancer cell invasion by preventing anterograde translocation and exocytosis of MT1-MMP. Moreover, when endosome translocation or exocytosis was inhibited by depletion of Protrudin or Synaptotagmin VII, respectively, invadopodia were unable to expand and elongate. Conversely, when Protrudin was overexpressed, noncancerous cells developed prominent invadopodia-like protrusions and showed increased matrix degradation and invasion. Thus, Protrudin-mediated ER-endosome contact sites promote cell invasion by facilitating translocation of MT1-MMP-laden endosomes to the plasma membrane, enabling both invadopodia outgrowth and MT1-MMP exocytosis.
Identifiants
pubmed: 32479595
pii: 151827
doi: 10.1083/jcb.202003063
pmc: PMC7401796
pii:
doi:
Substances chimiques
FYCO1 protein, human
0
Microtubule-Associated Proteins
0
SYT7 protein, human
0
Vesicular Transport Proteins
0
ZFYVE27 protein, human
0
rab7 GTP-Binding Proteins
0
rab7 GTP-binding proteins, human
0
Synaptotagmins
134193-27-4
MMP14 protein, human
EC 3.4.24.80
Matrix Metalloproteinase 14
EC 3.4.24.80
rab GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Pedersen et al.
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