Diagnostic accuracy of serum (1-3)-β-D-glucan for Pneumocystis jirovecii pneumonia: a systematic review and meta-analysis.


Journal

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
ISSN: 1469-0691
Titre abrégé: Clin Microbiol Infect
Pays: England
ID NLM: 9516420

Informations de publication

Date de publication:
Sep 2020
Historique:
received: 16 10 2019
revised: 19 05 2020
accepted: 20 05 2020
pubmed: 2 6 2020
medline: 3 7 2021
entrez: 2 6 2020
Statut: ppublish

Résumé

Pneumocystis jirovecii pneumonia (PJP) can be a life-threatening opportunistic infection in immunocompromised hosts. The diagnosis can be challenging, often requiring semi-invasive respiratory sampling. The serum 1,3-β-D-glucan (BDG) assay has been proposed as a minimally invasive test for the presumptive diagnosis of PJP. We carried out a systematic review and meta-analysis using articles in the English language published between January 1960 and September 2019. We estimated the pooled sensitivity and specificity of BDG testing using a bivariate random effects approach and compared test performance in human immunodeficiency virus (HIV) and non-HIV subgroups with meta-regression. Data from the pooled sensitivity and specificity were transformed to generate pre- and post-test probability curves. Twenty-three studies were included. The pooled sensitivity and specificity of serum BDG testing for PJP were 91% (95%CI 87-94%) and 79% (95%CI 72-84%) respectively. The sensitivity in patients with HIV was better than in patients without (94%, 95%CI 91-96%) versus 86% (95%CI 78-91%) (p 0.02), with comparable specificity (83%, 95%CI 69-92% versus 83%, 95%CI 72-90%) (p 0.10). A negative BDG was only associated with a low post-test probability of PJP (≤5%) when the pre-test probability was low to intermediate (≤20% in non-HIV and ≤50% in HIV). Among patients with a higher likelihood of PJP, the pooled sensitivity of BDG is insufficient to exclude infection. Similarly, for most cases, the pooled specificity is inadequate to diagnose PJP. Understanding the performance of BDG in the population being investigated is therefore essential to optimal clinical decision-making.

Sections du résumé

BACKGROUND BACKGROUND
Pneumocystis jirovecii pneumonia (PJP) can be a life-threatening opportunistic infection in immunocompromised hosts. The diagnosis can be challenging, often requiring semi-invasive respiratory sampling. The serum 1,3-β-D-glucan (BDG) assay has been proposed as a minimally invasive test for the presumptive diagnosis of PJP.
METHOD METHODS
We carried out a systematic review and meta-analysis using articles in the English language published between January 1960 and September 2019. We estimated the pooled sensitivity and specificity of BDG testing using a bivariate random effects approach and compared test performance in human immunodeficiency virus (HIV) and non-HIV subgroups with meta-regression. Data from the pooled sensitivity and specificity were transformed to generate pre- and post-test probability curves.
RESULTS RESULTS
Twenty-three studies were included. The pooled sensitivity and specificity of serum BDG testing for PJP were 91% (95%CI 87-94%) and 79% (95%CI 72-84%) respectively. The sensitivity in patients with HIV was better than in patients without (94%, 95%CI 91-96%) versus 86% (95%CI 78-91%) (p 0.02), with comparable specificity (83%, 95%CI 69-92% versus 83%, 95%CI 72-90%) (p 0.10). A negative BDG was only associated with a low post-test probability of PJP (≤5%) when the pre-test probability was low to intermediate (≤20% in non-HIV and ≤50% in HIV).
CONCLUSIONS CONCLUSIONS
Among patients with a higher likelihood of PJP, the pooled sensitivity of BDG is insufficient to exclude infection. Similarly, for most cases, the pooled specificity is inadequate to diagnose PJP. Understanding the performance of BDG in the population being investigated is therefore essential to optimal clinical decision-making.

Identifiants

pubmed: 32479781
pii: S1198-743X(20)30301-3
doi: 10.1016/j.cmi.2020.05.024
pii:
doi:

Substances chimiques

beta-Glucans 0

Types de publication

Journal Article Meta-Analysis Systematic Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

1137-1143

Informations de copyright

Copyright © 2020 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

Auteurs

Olivier Del Corpo (O)

Faculty of Medicine, McGill University, Montréal, Québec, Canada.

Guillaume Butler-Laporte (G)

Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada.

Donald C Sheppard (DC)

Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada; McGill Interdisciplinary Initiative in Infection and Immunity, Montréal, Canada; Department of Microbiology & Immunology, McGill University, Montréal, Canada.

Matthew P Cheng (MP)

Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada; McGill Interdisciplinary Initiative in Infection and Immunity, Montréal, Canada.

Emily G McDonald (EG)

McGill Interdisciplinary Initiative in Infection and Immunity, Montréal, Canada; Division of General Internal Medicine, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada; Clinical Practice Assessment Unit, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada.

Todd C Lee (TC)

Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada; McGill Interdisciplinary Initiative in Infection and Immunity, Montréal, Canada; Division of General Internal Medicine, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada; Clinical Practice Assessment Unit, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada. Electronic address: todd.lee@mcgill.ca.

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