Assessing efficacy of N-Acetyl-l-Cysteine-Sodium Hydroxide on bacterial viability and enhanced recovery of Mycobacterium avium subsp. paratuberculosis from bovine colostrum.
Acetylcysteine
/ chemistry
Animals
Cattle
Cattle Diseases
/ diagnosis
Cetylpyridinium
/ chemistry
Colostrum
/ microbiology
Decontamination
/ methods
Female
Microbial Viability
Mycobacterium avium subsp. paratuberculosis
/ isolation & purification
Paratuberculosis
/ diagnosis
Pregnancy
Sodium Hydroxide
/ chemistry
Bovine colostrum
Decontamination protocol
Improved recovery
Mycobacterium avium subsp. paratuberculosis (MAP)
N-Acetyl-(L)-Cysteine-Sodium Hydroxide (NALC-NaOH)
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
06
05
2020
revised:
28
05
2020
accepted:
28
05
2020
pubmed:
2
6
2020
medline:
22
7
2021
entrez:
2
6
2020
Statut:
ppublish
Résumé
The standard procedure for the improved cultural recovery of viable Mycobacterium spp. from diverse samples mainly depends on reducing the viability of background microbiota using different chemical compounds. This study was designed to i) evaluate the efficacy and comparison between N-Acetyl-l-Cysteine-Sodium hydroxide (NALC-2% NaOH) and hexadecylpyridinium chloride (0.75% HPC) treatment and exposure time on reducing the viability of undesirable microorganisms with minimal impact on colostrum consistency; and ii) assess the impact of NALC-2% NaOH on improved and enhanced recovery of Mycobacterium avium subsp. paratuberculosis (MAP) in spiked postpartum colostrum samples and consistency of colostrum. A total of 40 samples, each treated with NALC-2% NaOH for 15 min or 0.75% HPC for 5 h, were investigated for total mesophilic aerobic bacteria (MAB) and enterobacteria (EB) (CFU mL
Identifiants
pubmed: 32479871
pii: S0167-7012(20)30413-9
doi: 10.1016/j.mimet.2020.105968
pii:
doi:
Substances chimiques
Sodium Hydroxide
55X04QC32I
Cetylpyridinium
CUB7JI0JV3
Acetylcysteine
WYQ7N0BPYC
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105968Informations de copyright
Crown Copyright © 2020. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that there are no conflicts of interest.