Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents.
Antineoplastic Agents, Phytogenic
/ isolation & purification
Cell Line, Tumor
Cell Survival
/ drug effects
Coculture Techniques
Dose-Response Relationship, Drug
Humans
Inhibitory Concentration 50
Interleukin-6
/ metabolism
Lung Neoplasms
/ drug therapy
Neoplastic Stem Cells
/ drug effects
Rhodophyta
/ chemistry
Terpenes
/ isolation & purification
Tumor Microenvironment
Cancer stem cells
Interleukin-6
Lung cancer
Marine natural products
Microenvironment
Red seaweed
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
19
03
2020
revised:
09
05
2020
accepted:
15
05
2020
pubmed:
2
6
2020
medline:
27
2
2021
entrez:
2
6
2020
Statut:
ppublish
Résumé
Cancer is one of the major threats to human health and, due to distinct factors, it is expected that its incidence will increase in the next decades leading to an urgent need of new anticancer drugs development. Ongoing experimental and clinical observations propose that cancer cells with stem-like properties (CSCs) are involved on the development of lung cancer chemoresistance. As tumour growth and metastasis can be controlled by tumour-associated stromal cells, the main goal of this study was to access the antitumor potential of five bromoterpenes isolated from Sphaerococcus coronopifolius red alga to target CSCs originated in a co-culture system of fibroblast and lung malignant cells. Cytotoxicity of compounds (10-500 μM; 72 h) was evaluated on monocultures of several malignant and non-malignant cells lines (HBF, BEAS-2B, RenG2, SC-DRenG2) and the effects estimated by MTT assay. Co-cultures of non-malignant human bronchial fibroblasts (HBF) and malignant human bronchial epithelial cells (RenG2) were implemented and the compounds ability to selectively kill CSCs was evaluated by sphere forming assay. The interleucine-6 (IL-6) levels were also determined as cytokine is crucial for CSCs. Regarding the monocultures results bromosphaerol selectively eliminated the malignant cells. Both 12S-hydroxy-bromosphaerol and 12R-hydroxy-bromosphaerol steroisomers were cytotoxic towards non-malignant bronchial BEAS-2B cell line, IC
Identifiants
pubmed: 32480221
pii: S0753-3322(20)30467-4
doi: 10.1016/j.biopha.2020.110275
pii:
doi:
Substances chimiques
Antineoplastic Agents, Phytogenic
0
IL6 protein, human
0
Interleukin-6
0
Terpenes
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110275Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. All authors have approved the manuscript for submission and declare that this manuscript is original and unpublished, and has not been nor will be submitted to another journal for consideration unless it is rejected.