The DISC1-Girdin complex - a missing link in signaling to the T cell cytoskeleton.
Actin dynamics
DISC1
Dynein
Girdin
Immunological synapse
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
06 07 2020
06 07 2020
Historique:
received:
14
12
2019
accepted:
26
05
2020
pubmed:
3
6
2020
medline:
22
6
2021
entrez:
3
6
2020
Statut:
epublish
Résumé
In this study, using Jurkat cells, we show that DISC1 (disrupted in schizophrenia 1) and Girdin (girders of actin filament) are essential for typical actin accumulation at the immunological synapse. Furthermore, DISC1, Girdin and dynein are bound in a complex. Although this complex initially forms as a central patch at the synapse, it relocates to a peripheral ring corresponding to the peripheral supramolecular activation cluster (pSMAC). In the absence of DISC1, the classic actin ring does not form, cell spreading is blocked, and the dynein complex fails to relocate to the pSMAC. A similar effect is seen when Girdin is deleted. When cells are treated with inhibitors of actin polymerization, the dynein-NDE1 complex is lost from the synapse and the microtubule-organizing center fails to translocate, suggesting that actin and dynein might be linked. Upon stimulation of T cell receptors, DISC1 becomes associated with talin, which likely explains why the dynein complex colocalizes with the pSMAC. These results show that the DISC1-Girdin complex regulates actin accumulation, cell spreading and distribution of the dynein complex at the synapse.This article has an associated First Person interview with the first author of the paper.
Identifiants
pubmed: 32482796
pii: jcs.242875
doi: 10.1242/jcs.242875
pmc: PMC7358132
pii:
doi:
Substances chimiques
Actins
0
DISC1 protein, human
0
Microtubule-Associated Proteins
0
Nde1 protein, human
0
Nerve Tissue Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI118694
Pays : United States
Informations de copyright
© 2020. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
Références
Hum Mol Genet. 2008 Aug 15;17(16):2462-73
pubmed: 18469341
J Immunol. 2006 Dec 1;177(11):7707-14
pubmed: 17114441
Curr Biol. 2006 Jan 10;16(1):24-34
pubmed: 16401421
Nat Immunol. 2005 Jan;6(1):80-9
pubmed: 15558067
J Biol Chem. 2016 Jan 8;291(2):613-29
pubmed: 26553875
Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14883-8
pubmed: 16990435
J Cell Biol. 2015 Sep 28;210(7):1165-84
pubmed: 26391662
J Vis Exp. 2018 Jul 30;(137):
pubmed: 30102290
J Neurochem. 2014 Nov;131(4):457-69
pubmed: 25060559
Elife. 2017 Sep 26;6:
pubmed: 28949297
Development. 2016 Aug 1;143(15):2732-40
pubmed: 27287808
Immunity. 2007 Feb;26(2):177-90
pubmed: 17306570
Mol Psychiatry. 2007 Jan;12(1):74-86
pubmed: 17043677
Hum Mol Genet. 2011 Oct 15;20(R2):R175-81
pubmed: 21852244
PLoS One. 2019 Jul 3;14(7):e0210377
pubmed: 31269031
Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14418-23
pubmed: 17726106
Immunity. 2001 Mar;14(3):315-29
pubmed: 11290340
J Clin Invest. 2016 Oct 3;126(10):3837-3851
pubmed: 27599296
J Cell Biol. 2015 Feb 16;208(4):475-91
pubmed: 25666810
Elife. 2015 Mar 11;4:
pubmed: 25758716
Sci Signal. 2011 Sep 27;4(192):ra64
pubmed: 21954290
Nature. 1998 Sep 3;395(6697):82-6
pubmed: 9738502
Sci Rep. 2017 Jun 23;7(1):4156
pubmed: 28646160
J Immunol. 2016 Sep 15;197(6):2090-101
pubmed: 27534551
J Cell Biol. 2012 Jun 11;197(6):775-87
pubmed: 22665519
J Neuroimmune Pharmacol. 2015 Dec;10(4):610-9
pubmed: 26162591
J Biol Chem. 2005 May 6;280(18):18525-35
pubmed: 15753085
Front Cell Neurosci. 2014 Jul 08;8:190
pubmed: 25071449
J Biol Chem. 2016 Apr 8;291(15):8269-82
pubmed: 26887938
J Cell Sci. 2017 Aug 1;130(15):2435-2446
pubmed: 28701514
Neuron. 2009 Sep 24;63(6):774-87
pubmed: 19778507
J Exp Med. 2013 Nov 18;210(12):2721-37
pubmed: 24190432
J Immunol. 2011 Jun 1;186(11):6227-37
pubmed: 21525391
Autoimmunity. 2010 Feb;43(1):64-75
pubmed: 20001423
J Immunol. 2012 Jun 1;188(11):5421-7
pubmed: 22529300
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15873-8
pubmed: 19805229
Mol Cell Neurosci. 2004 May;26(1):112-22
pubmed: 15121183
Cell Prolif. 2016 Dec;49(6):740-750
pubmed: 27666957
J Immunol. 2019 Aug 1;203(3):760-768
pubmed: 31201236
PLoS One. 2012;7(10):e48041
pubmed: 23110170
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7767-72
pubmed: 12802007
Bioessays. 2016 Apr;38(4):379-93
pubmed: 26879989
J Cell Biol. 2016 Nov 7;215(3):383-399
pubmed: 27799367
Mol Biol Cell. 2014 Nov 5;25(22):3654-71
pubmed: 25187647
Annu Rev Immunol. 2019 Apr 26;37:201-224
pubmed: 30576253
Mol Med Rep. 2015 Oct;12(4):5086-92
pubmed: 26151295
Science. 2003 Oct 3;302(5642):103-6
pubmed: 14526080
Front Immunol. 2018 Jan 18;9:25
pubmed: 29403502
Curr Biol. 2018 Feb 19;28(4):489-502.e9
pubmed: 29398219
Mol Cell Biol. 2006 Oct;26(19):7130-44
pubmed: 16980616
Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12436-41
pubmed: 19617540
Cell. 2016 Mar 24;165(1):100-110
pubmed: 26924577
Nat Immunol. 2002 Oct;3(10):911-7
pubmed: 12244310
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14151-6
pubmed: 14612578
Immunity. 2002 Jan;16(1):111-21
pubmed: 11825570
Mol Psychiatry. 2008 Jan;13(1):36-64
pubmed: 17912248
Cancer Sci. 2018 Nov;109(11):3643-3656
pubmed: 30194792
Cancer Sci. 2010 Apr;101(4):836-42
pubmed: 20132219
Cell Calcium. 2016 Nov;60(5):309-321
pubmed: 27451384
Science. 1999 Jul 9;285(5425):221-7
pubmed: 10398592
Curr Biol. 2006 Jan 10;16(1):35-46
pubmed: 16401422
J Exp Med. 1987 Jun 1;165(6):1565-80
pubmed: 2953845
Dev Cell. 2005 Sep;9(3):389-402
pubmed: 16139227