MiR-4269 suppresses the tumorigenesis and development of pancreatic cancer by targeting ZEB1/OTX1 pathway.
OTX1
ZEB1
miR-4269
pancreatic cancer
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
26 06 2020
26 06 2020
Historique:
received:
09
01
2020
revised:
20
05
2020
accepted:
01
06
2020
pubmed:
3
6
2020
medline:
24
3
2021
entrez:
3
6
2020
Statut:
ppublish
Résumé
As one of the most prevalent malignant tumors, pancreatic cancer (PC) is a leading fatal cancer worldwide. Surging evidence has unraveled that miRNAs are involved in the occurrence and progression of multiple cancers, including PC. The tumor suppressor effects of miR-4269 have been certified in gastric carcinoma. However, the potential function of miR-4269 remains largely unclear, which drives us to identify the role of miR-4269 in PC development. In the present study, we determined the expression pattern of miR-4269 in PC cells and normal cells. Results of RT-qPCR analysis illuminated that miR-4269 expression level in PC cells was lower than that in normal cells. Functional assays demonstrated that up-regulation of miR-4269 obviously inhibited the proliferation, migration and invasion of PC cells. In order to elucidate the mechanism governing miR-4269 in PC, we carried out bioinformatics analysis and further experimental investigations. Our results validated that ZEB1 was a direct target of miR-4269. Additionally, ZEB1 activated the transcription of OXT1. More importantly, miR-4269 attenuated the expression level of OXT1 via targeting ZEB1. Ultimately, our findings confirmed that miR-4269 served as a cancer suppressor in PC through regulation of ZEB1/OTX1 pathway, which suggested that miR-4269 might represent a promising target for the clinical treatment of PC.
Identifiants
pubmed: 32484209
pii: 225115
doi: 10.1042/BSR20200010
pmc: PMC7286876
pii:
doi:
Substances chimiques
MIRN4269 microRNA, human
0
MicroRNAs
0
OTX1 protein, human
0
Otx Transcription Factors
0
ZEB1 protein, human
0
Zinc Finger E-box-Binding Homeobox 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 The Author(s).
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