In vitro and in vivo characterization of CPP and transferrin modified liposomes encapsulating pDNA.

Animals Blood-Brain Barrier / metabolism Brain / metabolism Cell-Penetrating Peptides / chemistry Endothelial Cells / metabolism Female Liposomes / chemistry Male Mice Plasmids / chemistry Rats Rats, Sprague-Dawley Transferrin / chemistry
Brain Liposome PFVYLI R9F2 Transferrin

Journal

Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142

Informations de publication

Date de publication:
08 2020
Historique:
received: 17 01 2020
revised: 04 05 2020
accepted: 21 05 2020
pubmed: 3 6 2020
medline: 16 7 2021
entrez: 3 6 2020
Statut: ppublish

Résumé

The limitations imposed on brain therapy by the blood-brain barrier (BBB) have warranted the development of carriers that can overcome and deliver therapeutic agents into the brain. We strategically designed liposomal nanoparticles encasing plasmid DNA for efficient transfection and translocation across the in vitro BBB model as well as in vivo brain-targeted delivery. Liposomes were surface modified with two ligands, cell-penetrating peptide (PFVYLI or R9F2) for enhanced internalization into cells and transferrin (Tf) ligand for targeting transferrin-receptor expressed on brain capillary endothelial cells. Dual-modified liposomes encapsulating pDNA demonstrated significantly (P < 0.05) higher in vitro transfection efficiency compared to single-modified nanoparticles. R9F2Tf-liposomes showed superior ability to cross in vitro BBB and, subsequently, transfect primary neurons. Additionally, these nanoparticles crossed in vivo BBB and reached brain parenchyma of mice (6.6%) without causing tissue damage. Transferrin receptor-targeting with enhanced cell penetration is a relevant strategy for efficient brain-targeted delivery of genes.

Identifiants

DOI: 10.1016/j.nano.2020.102225 PMID: 32485318 PMC: PMC7438306
pubmed: 32485318
pii: S1549-9634(20)30079-4
doi: 10.1016/j.nano.2020.102225
pmc: PMC7438306
mid: NIHMS1599464
pii:
doi:

Substances chimiques

Cell-Penetrating Peptides 0
Liposomes 0
Transferrin 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102225

Subventions

Organisme : NIA NIH HHS
ID : R01 AG051574
Pays : United States

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

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Auteurs

Bruna Dos Santos Rodrigues (B)

Department of Pharmaceutical Sciences, School of Pharmacy, College of Health Professions, North Dakota State University, Fargo, ND, USA.

Takahisa Kanekiyo (T)

Department of Neuroscience, Mayo Clinic, Jacksonville, FL, USA.

Jagdish Singh (J)

Department of Pharmaceutical Sciences, School of Pharmacy, College of Health Professions, North Dakota State University, Fargo, ND, USA. Electronic address: jagdish.singh@ndsu.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Barrière hémato-encéphalique › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Peptides de pénétration cellulaire › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › In vitro and in vivo characterization of CPP...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Métalloprotéines › Protéines de liaison au fer › Transferrines › Transferrine › In vitro and in vivo characterization of CPP...