The Effect of Oligomerization on A Solid-Binding Peptide Binding to Silica-Based Materials.
linker-protein G
oligomerization
quartz crystal microbalance with dissipation monitoring (QCM-D)
silica-based materials
silica-binding peptide
solid-binding peptide (SBP)
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
30 May 2020
30 May 2020
Historique:
received:
28
04
2020
revised:
11
05
2020
accepted:
12
05
2020
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
4
6
2020
Statut:
epublish
Résumé
The bifunctional linker-protein G (LPG) fusion protein comprises a peptide (linker) sequence and a truncated form of Streptococcus strain G148 protein G (protein G). The linker represents a multimeric solid-binding peptide (SBP) comprising 4 × 21-amino acid sequence repeats that display high binding affinity towards silica-based materials. In this study, several truncated derivatives were investigated to determine the effect of the SBP oligomerization on the silica binding function of LPG (for the sake of clarity, LPG will be referred from here on as 4 × LPG). Various biophysical characterization techniques were used to quantify and compare the truncated derivatives against 4 × LPG and protein G without linker (PG). The derivative containing two sequence repeats (2 × LPG) showed minimal binding to silica, while the truncated derivative with only a single sequence (1 × LPG) displayed no binding. The derivative containing three sequence repeats (3 × LPG) was able to bind to silica with a binding affinity of K
Identifiants
pubmed: 32486317
pii: nano10061070
doi: 10.3390/nano10061070
pmc: PMC7353425
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Nat Mater. 2002 Nov;1(3):169-72
pubmed: 12618805
BMC Bioinformatics. 2017 May 25;18(1):277
pubmed: 28545462
ACS Appl Mater Interfaces. 2016 May 18;8(19):11945-53
pubmed: 27119593
Biomacromolecules. 2014 Jul 14;15(7):2369-77
pubmed: 24892212
Sci Rep. 2016 Feb 23;6:21833
pubmed: 26903196
Chem Rev. 2017 Oct 25;117(20):12641-12704
pubmed: 28849640
ACS Nano. 2010 Jan 26;4(1):452-8
pubmed: 20038158
Adv Drug Deliv Rev. 2013 Oct;65(10):1357-69
pubmed: 23026637
Langmuir. 2008 May 6;24(9):4938-43
pubmed: 18363413
Protein Pept Lett. 2008;15(9):956-63
pubmed: 18991772
N Biotechnol. 2013 Jun 25;30(5):485-92
pubmed: 23238030
Biotechnol Prog. 2006 Mar-Apr;22(2):401-5
pubmed: 16599553
Biochem J. 1991 Mar 1;274 ( Pt 2):503-7
pubmed: 2006913
Anal Biochem. 2000 Dec 15;287(2):252-60
pubmed: 11112271
Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12491-6
pubmed: 15314214
Nat Biotechnol. 1997 Mar;15(3):269-72
pubmed: 9062928
Chem Commun (Camb). 2013 Jun 4;49(44):5088-90
pubmed: 23626989
Sci Rep. 2015 Jul 08;5:12019
pubmed: 26153855
Chirality. 2014 Sep;26(9):471-82
pubmed: 24890763
Biophys J. 2007 Sep 15;93(6):2162-9
pubmed: 17513349
Biochemistry. 1981 Jan 6;20(1):33-7
pubmed: 7470476
Biotechnol Bioeng. 2007 Apr 15;96(6):1023-9
pubmed: 17013933
Colloids Surf B Biointerfaces. 2011 Sep 1;86(2):359-63
pubmed: 21592750
Biointerphases. 2014 Dec;9(4):041002
pubmed: 25553877
Sci Rep. 2015 Jun 22;5:11476
pubmed: 26098304
J Am Chem Soc. 2009 Jul 22;131(28):9704-14
pubmed: 19552440
Eur J Biochem. 2003 Aug;270(16):3345-52
pubmed: 12899691
Langmuir. 2012 Jan 17;28(2):1408-17
pubmed: 22148960
Life (Basel). 2018 May 08;8(2):
pubmed: 29738443
Biomacromolecules. 2009 Feb 9;10(2):250-7
pubmed: 19072301
Biomolecules. 2019 Dec 18;10(1):
pubmed: 31861313
Trends Biotechnol. 2015 May;33(5):259-68
pubmed: 25796487
J Am Chem Soc. 2012 Aug 1;134(30):12547-56
pubmed: 22720657
Chem Rev. 2013 Mar 13;113(3):1904-2074
pubmed: 23432378
ACS Appl Mater Interfaces. 2016 Jul 20;8(28):18620-30
pubmed: 27355097
Nat Nanotechnol. 2011 Sep 11;6(10):675-82
pubmed: 21909083
ACS Nano. 2013 Nov 26;7(11):9632-46
pubmed: 24124916
Bioinformatics. 2006 Aug 15;22(16):1955-62
pubmed: 16787970
J Mol Biol. 1992 Dec 20;228(4):1219-34
pubmed: 1474588
Nat Mater. 2003 Sep;2(9):577-85
pubmed: 12951599
Nat Mater. 2009 Jul;8(7):543-57
pubmed: 19525947